Position: Home > Articles > Characteristics of carbon fixing bacteria community in tropical farmland soils with different abandoned years in Hainan Island
Southwest China Journal of Agricultural Sciences
2024,37
(8)
1771-1779
海南岛不同撂荒年限的热带农田土壤固碳细菌群落特征
作 者：
韩知序;赵志忠;陈红利;余玉莹
单 位：
海南师范大学地理与环境科学学院
关键词：
热带农田;固碳细菌;cbbL基因;群落结构;高通量测序
摘 要：
[目的]探讨热带地区不同撂荒年限对农田土壤固碳细菌群落的影响,为农业可持续管理提供理论依据和数据支撑.[方法]采用实时荧光定量PCR(Real-time quantitative PCR)和高通量测序技术,对海南岛中部地区农田、短期撂荒地(2<撂荒年限<5)和长期撂荒地(撂荒年限>5)土壤固碳细菌的基因丰度、群落多样性及其差异进行分析.[结果]研究区内土壤cbbL基因丰度介于3.11 ×107~2.84 ×108 copies/g干土,呈现农田>短期撂荒地>长期撂荒地的规律.与对照农田相比,长期撂荒地降低土壤固碳细菌Chao1指数、Observed species指数和Shannon指数.不同撂荒年限热带农田土壤固碳细菌的优势菌门为假单胞菌门(Pseudomonadota)和放线菌门(Actinomycetota),优势菌属为慢生根瘤菌属(Bradyrhizobium)、分枝杆菌属(Mycobacterium)、红假单胞菌属(Rhodopseudomonas)、红长命菌属(Rubrivivax)、芽绿菌属(Blastochloris)、诺卡氏菌属(Nocardia)、中慢生根瘤菌属(Mesorhizobi-um)、独岛菌属(Dokdonella)、固氮螺菌属(Azospirillum),且以兼性自养菌为主.不同撂荒年限的土壤固碳细菌群落结构变化总体呈现出按各撂荒年限聚集,3种不同撂荒年限的土壤共分析出33个差异标志物,其中农田17个,短期撂荒地5个,长期撂荒地11个.RDA结果显示,土壤固碳细菌群落结构受到土壤有机碳(SOC)、土壤水分含量(SWC)、土壤易氧化有机碳(ROC)、全磷(TP)、全氮(TN)、粗砂(Coarse sand)、pH和细砂(Fine sand)的显著影响.[结论]撂荒年限的增加降低了土壤固碳细菌的基因丰度,群落结构也发生变化,环境因子中SOC、SWC、ROC、TP和TN对土壤固碳细菌群落结构的影响较大.
译 名：
Characteristics of carbon fixing bacteria community in tropical farmland soils with different abandoned years in Hainan Island
关键词：
Tropical farmland%Carbon fixing bacteria%cbbL gene%Community structure%High throughput sequencing
摘 要：
[Objective]The paper aimed to explore the impact of different years of abandonment in tropical regions on soil carbon fixing bacte-rial communities in farmland,and provide theoretical basis and data support for sustainable agricultural management.[Method]Real time quantitative PCR and high-throughput sequencing technology were used to analyze the gene abundance,community diversity,and differences of soil carbon fixing bacteria in farmland,short-term abandoned land(25)in the central region of Hainan Island.[Result]The abundance of cbbL gene in the soil within the region ranged from 3.11 × 107-2.84 × 108 copies/g of dry soil,showing a trend of farmland>short-term abandoned land>long-term abandoned land.Compared with the control farmland,long-term abandoned land reduced the Chao1 index,Observed Specifications index and Shannon index of soil carbon fixing bacteria.The dominant phyla of carbon fixing bacteria in tropical farmland soils with different abandonment years were Pseudomonadota and Actinomycetota,and the dominant genera were Bradyrhizobium,Mycobacterium,Rhodopseudomonas,Rubrivivax,Blastochlolis,Nocardia,Meso-rhizobium,Dokdonella and Azospirillum,and most of them were facultative autotrophic bacteria.The overall changes in the community struc-ture of soil carbon fixing bacteria with different abandonment years showed clustering according to each abandonment year.A total of 33 dif-ferential markers were analyzed for three different abandonment years of soil,including 17 in farmland,5 in short-term abandonment land,and 11 in long-term abandonment land.The RD A results showed that the community structure of soil carbon fixing bacteria was significantly influ-enced by SOC,SWC,ROC,TP,TN,Coarse sand,pH and Fine sand.[Conclusion]The increase in abandoned years has reduced the gene a-bundance of soil carbon fixing bacteria,and the community structure has also changed.Environmental factors such as SOC,SWC,ROC,TP and TN have a significant impact on the community structure of soil carbon fixing bacteria.
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