当前位置: 首页 > 文章 > 发酵床垫料中高效纤维素降解菌的分离与筛选 农业资源与环境学报 2015 (04) 383-387
Position: Home > Articles > Isolation and Identification of Cellulose-Decomposing Microorganisms in Deep-litter System Journal of Agricultural Resources and Environment 2015 (04) 383-387

发酵床垫料中高效纤维素降解菌的分离与筛选

作  者:
王震;许丽娟;刘标;杜东霞;尹红梅;贺月林
单  位:
湖南省微生物研究院
关键词:
发酵床垫料;纤维素降解菌;酶活;鉴定
摘  要:
为获得高效纤维素降解菌,从发酵床垫料中分离出5株能降解纤维素的微生物菌株,对5株菌进行了透明圈和纤维素酶活性测定。结果表明:5株菌株均可在纤维素-刚果红平板上快速形成透明圈,其中菌株A3产酶活性最强,在接种后72 h达到125.47U·m L-1。对其16S r DNA序列进行分析,结合形态学观察和生理生化特征,将该菌鉴定为地衣芽孢杆菌(Bacillus licheniformis)。培养时间和温度对菌株的酶活力影响较大,各菌株的产酶最适温度为30~40℃。该芽孢杆菌分解纤维素能力强、适应能力高,在发酵床垫料堆肥中具有潜在的应用前景。
译  名:
Isolation and Identification of Cellulose-Decomposing Microorganisms in Deep-litter System
作  者:
WANG Zhen;XU Li-juan;LIU Biao;DU Dong-xia;YIN Hong-mei;HE Yue-lin;Hunan Institute of Microbiology;
关键词:
deep-litter system;;cellulose-decomposing microorganism;;enzyme activity;;identification
摘  要:
To obtain effective cellulose-degrading microorganisms, five cellulose-decomposing strains were isolated from deep-litter system and their enzyme activities were determined by DNS method. The results showed that all of the strains could form the clear zone rapidly on the cellulose-congo red medium. The strain A3 showed the highest enzyme activity and reached 125.47 U·m L-1in 72 h after inoculation. Based on the physiological, biochemical characterization and 16 S r DNA analysis, the strain A3 was identified as Bacillus licheniformis. The enzyme activity was influenced by culture temperature and time. The optimal temperature for cellulase activity was 30 ~40 ℃. Overall, with the high capacity to decompose the cellulose and adaptability to environment, the Bacillus strain would have a great potential to be applied in utilizing the cellulose.

相似文章

计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊