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农业科学与技术(英文)
2006,7
(4)
15-18
Position: Home > Articles > Sequence related amplified polymorphism (SRAP) reaction system optimization and its application
Agricultural Science & Technology
2006,7
(4)
15-18
Sequence related amplified polymorphism (SRAP) reaction system optimization and its application
作 者：
Li Li
单 位：
Changsha 410125;Key Laboratory of Molecular Breeding for Hybrid Rice; Hunan Hybrid Rice Research Centre; China
关键词：
SRAP analysis;DNA Extraction;optimization;application
摘 要：
In this study, three methods such as CTAB.SDS and Shanlichun methods were used to extract genomic DNA from the seedling of rape to find the best method. The principle, characters and application of SRAP were introduced. In order to obtain the optimalSRAP reaction system, the factors including concentrations of DNA, dNTP, etc. of reaction system were modified to better the system of rape. The result showed that the optimum concentrations were15ng DNA template, 0.2mM dNTP, 1.0 mu M primer and 2.0U Taqenzyme in this 25 mu L SRAP-PCR system.
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