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当前位置: 首页 > 文章 > 基于COⅠ基因序列的日本医蛭疑似种鉴定 西南大学学报(自然科学版) 2022 (10) 74-80
Position: Home > Articles > Identification of Suspected Species of Hirudo nipponica Based on COⅠ Gene Sequence Journal of Southwest University(Natural Science Edition) 2022 (10) 74-80

基于COⅠ基因序列的日本医蛭疑似种鉴定

作  者：
余米;苏胜齐;周梦;曹敏;封孝兰
单  位：
西南大学水产学院;重庆市药物种植研究所;特色生物资源研究与利用川渝共建重点实验室
关键词：
日本医蛭;细胞色素氧化酶Ⅰ亚基;物种鉴定
摘  要：
采用线粒体COⅠ(细胞色素氧化酶Ⅰ亚基，tochrome oxidasesubunit Ⅰ)基因序列鉴别日本医蛭及其常见疑似种，并分析其遗传多样性和亲缘关系.以日本医蛭及其疑似种为材料，提取线粒体DNA,扩增其COⅠ基因后进行测序，并计算遗传距离和聚类分析.结果显示，日本医蛭COⅠ序列长度为680 bp,碱基A,T,C,G质量分数分别为35.1%,28.7%,16.2%和20.0%,碱基AT质量分数为63.8%;疑似种YS1～YS10的碱基A,T,C,G平均质量分数分别为39.8%,29.4%,15.2%和14.9%,碱基AT质量分数为69.2%. 11种蛭类COⅠ序列中有569个一致位点，84个变异位点，其中，40个位点发生了碱基转换，44个位点发生了碱基颠换，转换与颠换的比值为0.90.日本医蛭与疑似种的遗传距离在0.159～0.166之间.基于COⅠ序列的DNA条形码技术可以很好地鉴定正品日本医蛭与其疑似种，本试验鉴定的疑似种均不是日本医蛭.
译  名：
Identification of Suspected Species of Hirudo nipponica Based on COⅠ Gene Sequence
作  者：
YU Mi;SU Shengqi;ZHOU Meng;CAO Min;FENG Xiaolan;Chongqing Institute of Medicinal Plant Cultivation;College of Fisheries, Southwest University;Bio-resource Research and Utilization Joint Key Laboratory of Sichuan and Chongqing;
关键词：
Hirudo nipponica;;COⅠ gene;;species identification
摘  要：
The mitochondrial COⅠ gene sequence was used to analyze the genetic diversity and relationship between Hirudo nipponica and its suspected species. The mitochondrial DNA of H. nipponica and its suspected species were extracted. The COⅠ gene were amplified and sequenced. Genetic distance and relationship were analyzed. The results showed that the sequence length of COⅠ of H. nipponica was 680 bp. The contents of A, T, C and G were 35.1%, 28.7%, 16.2% and 20.0%, respectively, with 63.8% of AT content. The average contents of A, T, C and G in YS1～YS10 were 39.8%, 29.4%, 15.2% and 14.9%, respectively, with 69.2% of AT content. There were 569 consensus sites, 84 variation sites in 11 Hirudo species. Among them, 40 loci had base conversion and 44 had transversions. The ratio of conversion versus transversion was 0.90. The genetic distance between H. nipponica and 10 suspected species ranged from 0.159 to 0.166. DNA barcoding technology based on COⅠ sequence can be used to identify authentic H. nipponica and its suspected species. All the suspected species identified in this study were not H. nipponica.

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