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Position: Home > Articles > Process Conditions Optimization for Antioxidant Peptide Preparation from Small Yellow Croaker by Response Surface Methodology FOOD SCIENCE 2011,32 (21) 165-170

小黄鱼抗氧化肽制备条件的响应面优化

作  者：
徐鑫;何佳易;刘国艳;蔡丽丽;魏晓蕊;徐娜
单  位：
扬州大学食品科学与工程学院
关键词：
小黄鱼;抗氧化肽;响应曲面法;DPPH自由基
摘  要：
目的:优化胰蛋白酶(PTN 6.0S)酶解小黄鱼制备抗氧化肽的条件。方法:以酶解产物对1,1-二苯基苦基苯肼(DPPH)自由基的清除率为指标,通过单因素试验及响应曲面法优化酶解条件。结果:酶解条件在pH7.0、温度52℃、时间19h、底物质量浓度4.2g/100mL,酶与底物比6‰时,产物对DPPH自由基的清除率达到最大值(46.90±0.3)%。响应面分析表明,酶解时间对产物的抗氧化活性有显著影响;温度与酶与底物比、时间与底物质量浓度、酶与底物比与底物质量浓度在酶解工艺中存在复杂的交互作用。
译  名：
Process Conditions Optimization for Antioxidant Peptide Preparation from Small Yellow Croaker by Response Surface Methodology
作  者：
XU Xin,HE Jia-yi,LIU Guo-yan,CAI Li-li,WEI Xiao-rui,XU Na (College of Food Science and Engineering,Yangzhou University,Yangzhou 225127,China)
关键词：
small yellow croaker;antioxidant peptides;response surface methodology;DPPH radical
摘  要：
Objective: To optimize trypsin(PTN 6.0S) hydrolysis conditions of small yellow croaker for preparing antioxidant peptides.Methods: The optimization was performed using one-factor-at-a-time method and response surface methodology based on DPPH radical scavenging activity.Results: The optimal hydrolysis conditions were substrate concentration of 4.2 g/100 mL,enzyme-to-substrate ratio of 6‰,hydrolysis time of 19 h and hydrolysis temperature of 52 ℃.Maximum DPPH scavenging rate was obtained under the system of pH 7.0,reaching(46.90 ± 0.3)%.Response surface analysis showed that hydrolysis time had a significant effect on antioxidant activity of products and that the interactions between hydrolysis temperature and enzyme-to-substrate ratio,between hydrolysis time and substrate concentration,and between enzyme-to-substrate ratio and substrate concentration were complicated.

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