Position: Home > Articles > Effect of Altitude Chronic Hypoxia on HIF-2α,EPO and in Yak and Migrated Cattle on Qinghai-Tibetan Plateau
Acta Agriculturae Boreali-occidentalis Sinica
2016,25
(4)
490-495
长期慢性低氧对牦牛和黄牛血液中HIF-2α、EPO水平及红细胞相关指标的影响
作 者：
刘凤云;李愈娴;胡琳;刘世明;祁生贵;杨蕾;唐永平;吴天一
单 位：
青海省高原医学科学研究院科技部高原医学研究重点实验室培育基地
关键词：
慢性低氧;牦牛;高山迁饲黄牛;血细胞;HIF-2α;EPO
摘 要：
为探讨牦牛和迁饲黄牛血液中红细胞(RBC)、血红蛋白(HB)、低氧诱导因子-2α(HIF-2α)、促红细胞生成素(EPO)等因子对高原适应和习服的不同特征及调控机理,按照海拔低于3 500m和高于3 500m两组采集青海地区的牦牛血样,同时采集高山迁饲黄牛(海拔2 500m)及低海拔黄牛(海拔1 300m)血液,采用全自动血液分析仪测定血液中红细胞、血红蛋白、红细胞比容(HCT)、平均红细胞血红蛋白量(MCH)等;采用双抗夹心酶标法测血液中EPO、HIF-2α含量。通过t检验及单因素方差分析法分析指标间的差异性,并分析各指标与HIF-2α、EPO的相关性。结果表明,海拔高于3 500m牦牛组的RBC、HB、HCT显著升高,而HIF-2α和EPO水平却未发生显著变化。与迁饲黄牛和低海拔黄牛相比,牦牛血液中的RBC明显降低,而MCH、HIF-2α和EPO显著升高。牦牛血液中EPO、RBC、HB、HCT、MCH水平与HIF-2α水平呈显著相关;迁饲黄牛血液中红细胞分布宽度(RDW-SD)与EPO水平呈显著相关;低海拔黄牛血液EPO、MCH水平与HIF-2α水平呈显著相关。推测:海拔升高引起的低氧是影响牦牛血液RBC、HB、HCT改变的主要因素;与黄牛相比,低RBC、低MCH、高HIF-2α、高EPO水平是牦牛适应低氧的主要特征,且牦牛血细胞各指标对血液中HIF-2α水平的改变更敏感。提示牦牛通过遗传进化成功适应高原环境。
译 名：
Effect of Altitude Chronic Hypoxia on HIF-2α,EPO and in Yak and Migrated Cattle on Qinghai-Tibetan Plateau
作 者：
LIU Fengyun;LI Yuxian;HU Lin;LIU Shiming;QI Shenggui;YANG Lei;TANG Yongping;WU Tianyi;National Key Laboratory of High Altitude Medicine,Qinghai High Altitude Medical Research Institute;
关键词：
Chronic hypoxia;;Yak;;Migrated cattle;;Blood cells;;HIF-2a;;EPO
摘 要：
In order to investigate the difference adaptive features of red blood cell's parameter and its correlation with serum HIF-2α and EPO between yak and migrated cattle on Qinghai-Tibetan plateau,the serum samples of yak were collected under 3 500 mand up 3 500 mrespectively in Qinghai,meanwhile the serum samples of migrated cattle on plateau(2 500m)and lowland cattle(1 300m)were also collected.The levels of hematocrit(HCT),mean corpuscular volume(MCV),mean red blood cell hemoglobin content(MCH)etc.in serum were measured by using fully automatic hematology analyzer,and the levels of hypoxia inducible factor-2a(HIF-2α )and erythropoietin(EPO)in serum were measured by using enzyme linked immunosorbent assay.We analyzed the differences of the above and its correlation with HIF-2α and EPO.We used one way analysis of variance(ANOVA)and independent-samples t-test.The results showed that the levels of RBC,HB,and HCT in >3 500 mgroup of yak increased significantly compared with other groups which live in <3 500 m.As compared to migrated cattle and lowland cattle,the serum level of RBC was decreased whereas the level of MCH,HIF-2α and EPO of yak were increased significantly,respectively.The levels of RBC,HB,HCT,and MCH were significantly correlated to levels of HIF-2α ,and a high correlation between HIF-2α and EPO in yak.The levels of RDW-SD was only correlated to the serum EPO activities in migrated cattle,whereas it was showed there were correlations between MCH and HIF-2α and between HIF-2α and EPO in lowland cattle.These results indicated that hypoxic caused by altitude take a key role in the changes of RBC,HB,HCT and RDW-CV levels in mountainous yaks.Compared with the migrated cattle and lowland cattle,lower levels of RBC,MCH and RDW-CV,and higher levels of HIF-2a and EPO were the characteristics of the yak to adapt to hypoxic environment of high altitude.The levels of RBC,HB,HCT,MCV,MCH and RDW-SD in serum of yak had more sensitive to the changes of HIF-2α .The results suggest that yaks are genetically and successfully adapted to high altitude.
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