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一株耐盐植物内生真菌的筛选与鉴定

作  者:
孙海彦;尹慧祥;陈惠琴;王琪;Marilyn Roossinck;Regina S Redman;Rusty Rodriguez;包晓东
关键词:
盐田;黑酵母;聚类进化树;高效液相色谱
摘  要:
[目的]研究盐田原生态系统中的耐盐植物内生真菌.[方法]对38株从海南省莺歌海盐场和东方盐场分离的菌株进行耐盐生长测试.[结果]发现了1株在8%Na Cl浓度下生长较好的真菌,并根据其形态特征,ITS扩增序列聚类进化树分析,显示其分类地位为黑酵母菌(Hortaea werneckii).经过高效液相色谱分析其代谢产物,发现该真菌在0、2%、4%、8%Na Cl浓度下的发酵产物均有所不同.[结论]盐田生境中存在特殊的植物内生真菌区系,其中黑酵母菌Hortaea werneckii具有一定代表性,有深入研究的价值.
作  者:
SUN Hai-yan;YIN Hui-xiang;CHEN Hui-qin;Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences;Hainan Key Laboratory of Tropical Microbe Resources;
单  位:
SUN Hai-yan%YIN Hui-xiang%CHEN Hui-qin%Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences%Hainan Key Laboratory of Tropical Microbe Resources
关键词:
Saltern;;Black yeast;;Phylogenic tree;;HPLC
摘  要:
[Objective]To study the endophytic fungi of halotolerant in the original ecosystem of saltern. [Method]Salt stress tests were conducted on the endophytic fungi isolated from Yingehai Saltern and Dongfang Saltern in Hainan Province,to discover the halotolerant fungi among them. [Result]One isolate was further identified according to morphological and ITS sequence-based phylogenic analyses,which suggested it belongs to Hortaea werneckii,a black yeast species,in taxonomy. The ferment substance under 0,2%,4%,8% of sodium chloride of this isolate was studied with HPLC finger printing approach,and differential metabolites according to salt concentrations were observed. [Conclusion]The solar salterns may be occupied by idiographic microflora of endophytes,included Hortaea werneckii observed in this study,which maybe representative of further studies.

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