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当前位置: 首页 > 文章 > 苹果矮化砧木新品系“矮砧6号”茎尖组培快繁研究 北方园艺 2014 (22) 102-104
Position: Home > Articles > Study on Rapid-Micropropagation Technique by Shoot Tip Culture of New Apple Dwarf Rootstock Strain‘Short Stock No.6' Northern Horticulture 2014 (22) 102-104

苹果矮化砧木新品系“矮砧6号”茎尖组培快繁研究

作  者：
王淼淼;马晓月;张学英;徐继忠
单  位：
河北农业大学园艺学院
关键词：
苹果;矮化砧木;茎尖培养;组培快繁;植物生长调节剂
摘  要：
以苹果矮化砧木新品系"矮砧6号"茎尖为外植体,研究了培养基中不同植物生长调节剂配比对芽苗增殖和生根的影响,以筛选出适合"矮砧6号"茎尖组培快繁的培养基配方。结果表明:苹果矮化砧木"矮砧6号"茎尖用0.1%HgCl2杀菌处理的适宜时间为8min;以MS为基本培养基,附加蔗糖30g/L、琼脂6g/L,适宜增殖培养的植物生长调节剂配比为6-BA 1.0mg/L+NAA0.05~0.10mg/L,增殖系数可达到每4周4.53倍;有利于生根的继代培养基的植物生长调节剂配比为6-BA 1.0mg/L+NAA 0.1mg/L,适宜组培苗生根的植物生长调节剂为IAA 1.0mg/L+IBA0.4mg/L,平均生根率为90%以上,平均生根数为6条左右,根系发达,移栽后成活率达90%以上。
译  名：
Study on Rapid-Micropropagation Technique by Shoot Tip Culture of New Apple Dwarf Rootstock Strain‘Short Stock No.6'
作  者：
WANG Miao-miao;MA Xiao-yue;ZHANG Xue-ying;XU Ji-zhong;College of Horticultural,Hebei Agricultural University;
关键词：
apple;;dwarf rootstock;;shoot tip culture;;rapid-micropropagation;;plant growth regulator
摘  要：
Taking the new strain‘Short Stock No.6'of apple dwarf rootstock as materials,the effects of plant growth regulators on multiplication and rooting of seedlings in vitro were researched to selecte the optimal medium.The results showed that the sterilization time of shoot tip using 0.1% HgCl2 was 8 minutes.The basic medium was MS supplemented with sugar 30g/L and agar 6g/L,the optimal plant growth regulators in subculture medium were 6-BA1.0mg/L+NAA 0.05-0.10mg/L,and the coefficient of multiplication reached 4.53 times every four weeks.The optimal plant growth regulators of subculture medium that suited rooting were 6-BA 1.0mg/L+NAA 0.1mg/L,the optimal plant growth regulators for rooting were IAA 1.0mg/L+IBA 0.4mg/L,the rooting rate was more than 90%,and the average number of root was about 6.Then the rooted plantlets were transplanted,the survival rate was more than90%.

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