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当前位置: 首页 > 文章 > FQ-PCR检测肌注小鹅瘟病毒VP3基因疫苗在雏鹅体内的动态分布 四川农业大学学报 2007,25 (4) 93-98
Position: Home > Articles > Dynamic Distribution of GPV-VP3 Gene Vaccine in Geese via Vaccinated with Intramuscular Injection Journal of Sichuan Agricultural University 2007,25 (4) 93-98

FQ-PCR检测肌注小鹅瘟病毒VP3基因疫苗在雏鹅体内的动态分布

作  者：
黎敏;程安春;汪铭书;韩新锋;刘晓东;卢菲;车茜;陈孝跃
单  位：
四川农业大学动物医学院禽病防治中心
关键词：
FQ-PCR;pcDNA-GPV-VP3;肌肉注射;雏鹅;动态分布
摘  要：
将30日龄四川白鹅分别肌肉注射接种50μg、100μg和200μg的GPV-VP3基因疫苗(pcDNA-GPV-VP3),以生理盐水和空载体质粒pcDNA3.1(+)为对照,于免疫接种后1h、12h、1d、3d、7d、21d、35d、63d、105d和217d采集全血以及各组织器官,用实时荧光定量PCR(FQ-PCR)检测pcDNA-GPV-VP3在雏鹅体内的动态分布。结果表明:①pcDNA-GPV-VP3各剂量免疫雏鹅1h即可在各组织中被检测到,其中注射部位含量最高,肝、淋巴器官(脾、法氏囊、胸腺、哈氏腺)含量较高;②到217d时,50μg组免疫雏鹅各个组织器官内仍检测到pcDNA-GPV-VP3的存在,但多数组织器官中的含量比1h时约少了103～104;③血液中pcDNA-GPV-VP3的含量较少,且1h～217d各时间点的差异不显著(P≥0.05);④不同剂量pcDNA-GPV-VP3免疫雏鹅各组织中的含量呈现的总体规律为200μg组>100μg组>50μg组,但差异不显著(P≥0.05)。因此,pcDNA-GPV-VP3免疫雏鹅后1h时可分布至雏鹅体内各组织器官中并持续存在217d以上。
译  名：
Dynamic Distribution of GPV-VP3 Gene Vaccine in Geese via Vaccinated with Intramuscular Injection
作  者：
LI Min1,CHENG An-chun1,2,WANG Ming-shu1,2,Han Xin-feng1,LU Fei1,CHE Qian1,LIU Xiao-dong1,Chen Xiao-yue1(1.Avain Disease Research Center,College of Veterinary Medicine,Sichuan Agriculture University;2.Key Laboratory of Animal Disease and Human Health of Sichuan Province,Yaan 625014,Sichuan,China)
关键词：
FQ-PCR;pcDNA-GPV-VP3;intramuscular;geese;dynamic distribution
摘  要：
This paper concentrates on 30-day-old geese were administered with 50 μg,100 μg,200 μg of pcDNA-GPV-VP3 respectively by the route of intramuscular injection with Normal saline,pcDNA3.1(+) as control.To detect the recombinant plasmid in the samples of peripheral blood and inoculated muscle,heart,liver,spleen,lung,kidney,brain,thymus,gland,harderian gland,bursa of fabricius,pancreas,duodenum,jejunum,ileum,rectum,appendices and serum with FQ-PCR at the interval of 1 h,12 h,1 d,3 d,7 d,21 d,35 d,63 d,105 d and 217 d after administration.Results showed that:①The pcDNA-GPV-VP3 could be detected in all tissues and peripheral blood at 1 h post infection.The copy number was the cargese at the skin of injected spot,then come liver,and lymphatic organ(spleen,fabricius,thymus,gland of harder);②At 217 d post administration,pcDNA-GPV-VP3 could still be detected in tissues of 50 μg dose group.But the copy number decreased about 103～104 copies in the most tissues than it was at 1 h post administration;③The changement of the copy numbers in the serum was always small and not obvious with the time(P≥0.05);④The quantity of pcDNA-GPV-VP3detected in the experimental groups showed the relations with the quantity of the inoculation doses,but not proportion(P≥0.05).The results demonstrated that pcDNA-GPV-VP3 could distribute in all tissues of geese at at 1 h post infection and existed in those after 217 d post infection.

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