当前位置: 首页 > 文章 > 黄瓜性别决定基因相关的分子标记 湖南农业大学学报(自然科学版) 2008,34 (4) 403-408
Position: Home > Articles > On the molecular markers of sex determining genes in cucumber Journal of Hunan Agricultural University(Natural Sciences) 2008,34 (4) 403-408

黄瓜性别决定基因相关的分子标记

作  者:
刘晓虹;陈惠明;许亮;易克;卢向阳
单  位:
湖南省蔬菜研究所;湖南农业大学
关键词:
黄瓜;分子标记;性别决定基因;黄瓜ACC合酶1G基因
摘  要:
以不同性别类型的黄瓜高代自交系为材料,根据GenBank中提供的乙烯合成、信号转导有关基因序列、花器官发育相关的黄瓜性别决定蛋白基因、AGMOUS同源异型基因、与性别表达相关的RAPD和ISSR引物,采用Primer Premier 5.00专业引物设计软件,设计可能与性别表达有关特异引物.结果表明,ACS1号引物对(ACS-F1,5′-ATTCAGATGGGT-3′;ACCS-R1,5′-GCCAAAGCTCGACAT-3′),CsACS1G-SCAR引物对(ACS-F1,5′-TTAACTACTTCGGACGGGCATAG-3′;ACCS-R1,5′-AGGTGTTCAGCAAA CATAGGGTG-3′)及RAPD引物S12对不同性别类型的黄瓜基因组DNA的PCR扩增产物表现出明显的多态性.用Mapmaker/Exp 3.0进行连锁分析,发现雌性F基因和ACS_280,CsACS1G-SCAR_430均连锁群,遗传距离分别为31.4,10.0 cM,与S12_280标记不在一个连锁群上.
译  名:
On the molecular markers of sex determining genes in cucumber
作  者:
LIU Xiao-hong1,CHEN Hui-ming1,2,XU Liang2,YI Ke2,LU Xiang-yang2(1.Hunan Engineering Research Center for Vegetable,Hunan Vegetable Research Institute,Changsha 410125,China;2.College of Bioscience and Technology,HNAU,Changsha 410128,China)
关键词:
cucumber;molecular maker;sex determining gene;CsACS1G
摘  要:
Genome DNA of different sexual cucumber inbreed lines was amplified by several RAPD,ISSR primers and sequence-specific primers which were designated by Primer Premier 5.00 from DNA sequence of ethylene biosynthesis and ethylene signal transduction pathway,sex determining protein,AGMOUS homologs with the identity of sex organ.The results indicated that two sequence-specific pair primers(ACR pair primers:ACS-F1,5′-ATTCAGATGGGT-3′;ACS-R1,5′-GCCAAAGCTCGACAT-3′;CsACS1G-SCAR primers:ACS-F1,5′-TTAA CTACTTCGGACGGGCATAG-3′;ACS-R1,5′-AGGTGTTCAGCAAACATAGGGTG-3′)and S12 RAPD primer have polymorphisms which were related to sex expression.Mapmaker/Exp 3.0 was used to study the linkage between makers and F gene.Through linkage analysis,F gene was found to be linked with ACS_280,CsACS1G-SCAR_430 whose distances were 31.4,10 cM respectively.

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