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当前位置: 首页 > 文章 > 平邑甜茶后代双胚苗的倍性鉴定及核型分析 果树学报 2009,26 (2) 129-134
Position: Home > Articles > Ploidy identification and karyotype analysis on the seedlings from di-embryonic seeds of Pingyi Tiancha(Malus hupehensis var.pingyiensis) Journal of Fruit Science 2009,26 (2) 129-134

平邑甜茶后代双胚苗的倍性鉴定及核型分析

作  者：
王颖;周攀;魏鑫;王玉霞;董文轩
单  位：
沈阳农业大学园艺学院;沈阳区域气候中心;辽宁省果树科学研究所
关键词：
平邑甜茶;双胚苗;染色体倍性;核型分析
摘  要：
为了确定平邑甜茶双胚苗的倍性特征和核型特点,采用压片和流式细胞仪观测的方法对平邑甜茶后代中双胚苗进行染色体倍性观察和核型分析。结果表明:4株供试材料均为三倍体,2n=3x=51,染色体为小型,染色体长度比差异不大;核型组成由中部着丝点染色体(m)和亚中部着丝点染色体(sm)组成;株系8-1、8-2、12-1和12-2的核型公式分别为2n=3x=45m+6sm,2n=3x=48m+3sm,2n=3x=27m+24sm,2n=3x=33m+18sm;染色体相对长度和着丝点指数的变异范围分别为1.21%～2.68%和34.19%～42.37%,1.44%～2.63%和35.59%～44.08%,1.36%～3.02%和32.85%～42.18%,1.43%～2.87%和34.23%～44.02%;核型分别属于1B、1A、2B和1B类型,表现为株系之间的核型不完全相同。本研究为丰富无融合生殖型砧木的育种理论,获得新的苹果无融合生殖型砧木提供有益的借鉴。
译  名：
Ploidy identification and karyotype analysis on the seedlings from di-embryonic seeds of Pingyi Tiancha(Malus hupehensis var.pingyiensis)
作  者：
WANG Ying1,ZHOU Pan2,WEI Xin2,WANG Yu-xia1,DONG Wen-xuan1(1College of Horticulture,Shenyang Agricultural University,Shenyang,Liaoning 110161 China;2Liaoning Research Institute of Pomology,Xiongyue,Liaoning 115009 China)
关键词：
Pinyi Tiancha(Malus hupehensis var.pingyiensis);Di-embryonic seedlings;Chromosome ploidy;Karyotype analysis
摘  要：
Using the seedlings from di-embryonic seeds of Pingyi Tiancha(Malus hupehensis var.pingyiensis) as materials,the chromosome number was observed and the karyotype was analysed by the methods of Squash and Cytometer.The results indicated that the ploidy of observed 4 seedlings(8-1,8-2,12-1 and 12-2) were all triploidy and composed of metacentric chromosomes and submetacentric chromosomes:2n=3x=51 which were all small chromosomes and whose morphological vari-ations and relative length ratios were not obvious.The karyotype formulas of them were 2n=3x=45m+6sm,2n=3x=48m+3sm,2n=3x=27m+24sm and 2n=3x=33m+18sm,respectively.The ranges of variation in the chromosome relative length and cen-tromere index were 1.21% to 2.68% and 34.19% to 42.37%,1.44% to 2.63% and 35.59% to 44.08%,1.36% to 3.02% and 32.85% to 42.18%,1.43% to 2.87% and 34.23% to 44.02%,respectively.The karyotypes of the 4 seedlings were 1B,1A,2B and 1B,respectively,which indicated that the karyotypes were not identical completely among the 4 seedlings.This re-search enriched the breeding methods and offered some new apomictic stocks in Malus for the utilization in fruit production.

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