Position: Home > Articles > Genetic Polymorphism Analysis of KAP7 Gene in 3 Goat Breeds
China Animal Husbandry & Veterinary Medicine
2013,40
(7)
133-136
3个山羊品种角蛋白辅助蛋白7基因的遗传多态性分析
作 者：
彭艺艺;杨朝霞;耿荣庆;陈玉林;张富全;张西顺
单 位：
阿克苏山羊研究中心;西北农林科技大学动物科技学院
关键词：
角蛋白辅助蛋白7基因;PCR-SSCP;陕北白绒山羊;太行黑山羊;南疆绒山羊
摘 要：
本研究采用PCR-SSCP方法对陕北白绒山羊、太行黑山羊及南疆绒山羊的角蛋白辅助蛋白(keratin-associated pro-teins,KAP)7基因进行遗传多态性研究。结果显示,KAP7基因在3个山羊品种中有多态性,有AA和AB 2种基因型,AA基因型均为其优势基因型。测序结果发现,KAP7基因在129bp处存在C→G的突变,突变的位置位于5′调控区。
译 名：
Genetic Polymorphism Analysis of KAP7 Gene in 3 Goat Breeds
作 者：
PENG Yi-yi1,YANG Zhao-xia1,GENG Rong-qing1,CHEN Yu-lin1, ZHANG Fu-quan2,ZHANG Xi-shun2(1.College of Animal Science and Technology,Northwest A&F University,Yangling 712100,China; 2.Goat Research Center of Akesu in Xinjiang Province,Aksu 843000,China)
关键词：
KAP7 gene;PCR-SSCP;Shanbei White cashmere goat;Taihang Black goat;Nanjiang White cashmere goat
摘 要：
The polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) was used to detect the polymorphism of keratin associated protein 7(KAP7) in Shanbei White cashmere goat,Taihang Black goat and Nanjiang White cashmere goat.The results showed that the KAP7 presented AA and AB genetypes in these three breeds,AA genetype was the dominant genetypes.The PCR production of different genotype of KAP7 were purified and sequnenced.One mutation,129 bp(C→G) was found in KAP7.This mutation site was in 5′ regulatory region.
导出引用
计量
文章访问数: 15
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
