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当前位置: 首页 > 文章 > 黄体酮对斑马鱼胚胎生长发育轴基因表达的影响 湖南农业科学 2019 (5) 81-84
Position: Home > Articles > Effects of Progesterone on the mRNA Expression of Genes Involved in Growth Axis in Embryonic Zebrafish Hunan Agricultural Sciences 2019 (5) 81-84

黄体酮对斑马鱼胚胎生长发育轴基因表达的影响

作  者：
梁燕秋;岑美祈;李进;郭慧
单  位：
广东海洋大学
关键词：
孕激素;黄体酮;下丘脑-垂体-生长轴;基因表达;斑马鱼
摘  要：
为了研究黄体酮对水生生物的生态毒理效应,以斑马鱼为试验材料,研究黄体酮对斑马鱼早期胚胎发育阶段下丘脑-垂体-生长轴相关基因转录表达的影响.将斑马鱼胚胎分别暴露在含0、6、45和90 ng/L的黄体酮水环境中144 hpf(hours post fertilization,受精后).结果显示:黄体酮在不同暴露时间点可以调节生长激素释放激素(ghrh)基因的转录表达,可以诱导生长激素(gh)和胰岛素生长因子1受体a(igf1ra)基因的转录表达,但抑制了胰岛素生长因子2(igf2a、igf2b)和胰岛素生长因子1受体b(igf1rb)基因的转录表达.此外,黄体酮暴露对生长激素受体(ghra、ghrb)和胰岛素生长因子1(igf1)基因的转录表达没有显著影响.试验表明黄体酮能够改变斑马鱼早期胚胎发育阶段下丘脑-垂体-生长轴相关基因的转录表达水平,进而对斑马鱼的生长发育具有潜在的危险.
译  名：
Effects of Progesterone on the mRNA Expression of Genes Involved in Growth Axis in Embryonic Zebrafish
作  者：
LIANG Yan-Qiu;CEN Mei-Qi;LI Jin;GUO Hui;Guangdong Ocean University;
关键词：
progestin;;progesterone;;hypothalamic-pituitary-growth axis;;gene expression;;zebrafish
摘  要：
Progesterone is widely present in surface waters and wastewaters from farms. In order to assess the adverse effects of progesterone on aquatic organisms, zebrafish(Danio rerio) embryos as experimental organism were exposed to 0, 6, 45 and 90 ng/L progesterone for 144 hpf(hours post fertilization) to conduct the gene transcriptional alterations along the hypothalamic-pituitary-growth axis. The results showed that progesterone could mediate mRNA expression of growth hormone-releasing hormone(ghrh) gene at different exposure time points. Progesterone exposure led to a significant up-regulation of growth hormone(gh) and insulin-like growth factor 1 receptor alpha(igf1 ra) genes. However, transcriptional expression of insulin-like growth factor 2(igf2 a, igf2 b) and insulin-like growth factor 1 receptor beta(igf1 rb) genes were inhibited by progesterone. In addition, progesterone treatment had no significant effects on the transcriptional profiles of target genes associated with growth hormone receptor(ghra, ghrb) and insulin-like growth factor 1(igf1) genes.Taken together, the overall results demonstrated that progesterone could significantly affect transcriptional expression levels of genes related to hypothalamic-pituitary-growth axis, which may pose potential adverse effects to the growth of zebrafish.

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