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Position: Home > Articles > Effect of UV-B Radiation on Gene Expression in Peels of‘Liangping’Pommelo[Citrus grandis(L.)Osbeck] Acta Horticulturae Sinica 2011,38 (5) 859-866

紫外线照射对梁平柚果皮基因表达的影响

作  者：
程春振;朱世平;吴波;阳佳位;贝学军;马岩岩;钟广炎
单  位：
西南大学园艺园林学院
关键词：
柑橘;紫外辐射;抑制性差减杂交;表达性序列标签;基因聚类分析
摘  要：
采用SSH技术以紫外照射的梁平柚[Citrus grandis(L.)Osbeck]果皮作为实验方(tester),以未被照射的正常果皮作为驱动方(driver),构建了一个梁平柚果皮紫外诱导基因的正向差减文库。经菌液PCR检测后随机挑取200个阳性克隆测序,获得168条表达序列标签(ESTs)。比对这168条ESTs,发现有分属于57个基因的114条ESTs与已知基因高度同源,54条ESTs同源性较低或没有同源性。功能分析发现,这些ESTs主要参与抗逆防御、生长发育、细胞凋亡、转录与翻译、细胞分化、信号传导、能量代谢、糖类及氨基酸代谢以及次生代谢等。
译  名：
Effect of UV-B Radiation on Gene Expression in Peels of‘Liangping’Pommelo[Citrus grandis(L.)Osbeck]
作  者：
CHENG Chun-zhen1,2,ZHU Shi-ping1,2,WU Bo1,2,YANG Jia-wei1,3,BEI Xue-jun1,2,MA Yan-yan1,2,and ZHONG Guang-yan1,2, (1College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400716,China;2Citrus Research Institute,Chinese Academy of Agricultural Sciences/ Southwest University,Chongqing 400712,China;3Kaixian Fruit Technology Extension Station,Chongqing 405400,China)
关键词：
citrus;ultravolet radiation;suppression subtractive hybridization;expressed sequence tags;gene ontology
摘  要：
A suppression subtractive hybridization library was successfully constructed using cDNA synthesized from RNA extracted from peels of‘Liangping’pemmelo[Citrus grandis(L.)Osbeck] receiving UV-B radiation as tester and non-UVB treatment as driver. Two hundred randomly-picked positive clones were sequenced,and a total of 168 EST sequences were obtained. One hundred and fourteen ESTs,involving in 57 genes,were found to share considerable homology with known genes while the rest 54 ESTs had low or even no homology with known genes. Function analysis showed that these differentially expressed genes were related to numerical celluar events such as stress-tolerance,growth and development,cell apoptosis,cell differenciation,gene transcription and translation,signaling,energy metabolism,carbohydrate and amino acid metabolism and secondary metabolism.

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