Haigang Bao;Han WenPeng;Ze XiaoLe

†College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;†College of Animal Science and Technology, China Agricultural University, Beijing 100193, Chin

horses;polymorphism;g212;thoroughbred;baise;site

[Objective] The aim was to study polymorphism of mat-peptide sequence of IGF1R gene in Baise horses and thoroughbred,which would afford reference for the further studies on the dwarf mechanism and molecular breeding in horses.[Method] A total of 57 blood samples of each breed were collected and genomic DNA was extracted by the standard phenol-chloroform method.Five DNA pools of each breed were constituted and polymorphism sites were identified by sequencing PCR products.Frequencies of genotypes and alleles at these sites of each breed were checked by PCR-RFLP.[Result] Four polymorphism sites were identified in exon 2,5 and 16,including mutations of T406C,T179 627C,G212 077A and G212 110A.No difference was found in the frequency of T179 627C between the Baise horses and thoroughbred.The mutation G212 077A was only found in the thoroughbred,and the mutations,T406C and G212 110A,were only checked out in the Baise horses.[Conclusion] Whether these mutations are associated with horse growth needs further studies.