Position: Home > Articles > Studies on Viability and Storage Capacity of Pollens from Two Tea Cultivars
Hubei Agricultural Sciences
2013,52
(24)
125-129
两种茶树花粉的生活力及贮藏力研究
作 者：
许林;李应发;周忠诚;李卓;杜克兵
单 位：
湖北生态工程职业技术学院;河南农业大学林学园艺学院;华中农业大学园艺林学学院
关键词：
台茶12号(C.sinensis‘Taicha 12’);福鼎大白茶(C.sinensis ‘Fuding-dabaicha’);花粉;离体萌发;生活力;贮藏力
摘 要：
为开展茶树[Camellia sinensis(L.)O.Kuntze]杂交育种工作,以茶树品种台茶12号(C.sinensis‘Taicha 12’)和福鼎大白茶(C.sinensis‘Fuding-dabaicha’)为试材,采用离体培养法开展花粉生活力与贮藏力研究。结果表明,H3BO3、蔗糖、pH、琼脂浓度以及萌发温度对供试的两种茶树花粉离体发芽率均有明显影响。两种茶树花粉离体萌发的最适培养基成分均为300 mg/L Ca(NO3)2+200 mg/L MgSO4+100mg/L KNO3+100 mg/L H3BO3+10%蔗糖+1.0%琼脂,最适pH 5.4,适宜萌发温度为16~21℃。贮藏温度对茶树花粉生活力保持具有显著影响,温度越低越有利于花粉活力的保持。-70℃最适于茶树花粉的长期贮藏,贮藏180 d后,台茶12号、福鼎大白茶的花粉发芽率分别为71.12%、50.61%。在生产实践中,-20℃贮藏茶树花粉亦可取得较好的效果,贮藏180 d后,台茶12号、福鼎大白茶的花粉发芽率分别为60.16%、46.88%。
译 名：
Studies on Viability and Storage Capacity of Pollens from Two Tea Cultivars
作 者：
XU Lin;LI Ying-fa;ZHOU Zhong-cheng;LI Zhuo;DU Ke-bing;College of Horticulture & Forestry Sciences,Huazhong Agricultural University;Wuhan Forestry & Ftuit Tree Research Institute;Hubei Ecology Vocational College;College of Forestry and Horticulture Sciences,Henan Agricultural University;
关键词：
Camellia sinensis ‘Taicha 12';;Camellia sinensis ‘Fuding-dabaicha';;pollen;;germination in vitro;;viability;;storage capacity
摘 要：
For tea hybridization, viability and storage capacity of pollens from Camellia sinensis ‘Taicha 12' and ‘Fuding- dabaicha' were studied using culture in vitro. Results showed that H3 BO3, sucrose, pH, agar concentration and germinating temperature had significant effects on germination rate of the pollens tested. The optimum medium of both pollens was 300 mg / L Ca(NO3)2 + 200 mg / L MgSO4 + 100 mg / L KNO3 + 100 mg / L H3 BO3 + 10% sucrose +1.0% agar, pH 5.4. The best temperature was 16~21 ℃. Lower storage temperature was suitable for keeping tea pollens viability.-70 ℃ was suitable for long-time storage. Stored for 180 d, pollens from ‘Taicha 12' and ‘Fuding-dabaicha' had germination rate of 71.12% and 50.61%, respectively. Stored under-20 ℃ for 180 d, pollens from ‘Taicha 12' and ‘Fuding-dabaicha' had germination rate of 60.16% and 46.88%, respectively.
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