Mycorrhizal Fungus rDNA ITS and Specificity of Cypripedium yunnanens and Cypripedium guttatum

ZHAO Xin-Yu;MIAO Fu-Jun;LI-Lu;WANG Juan;Southwest Forestry University;Yunnan Academy of Forestry;Yunnan Laboratory for Conservation of Rare,Endangered & Endemic Forest Plants,Public Key Laboratory of the State Forestry Administration;Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants;

Cypripedium yunnanense;;C.guttatum;;rDNA ITS;;mycorrhizal fungus;;specificity

Wild lady's slipper orchids( Cypripedium calceolus) as an ornamental plant with highly medicinal value,are being seriously threatened due to over collection,and are becoming endangered species. For a success cultivation and breeding of Cypripedium,mycorrhizal fungus is playing a very important role in this process. In this test,mycorrhizal fungus extracted from C. yunnanense and C. guttatum which distributed in northwest Yunnan,were studied through method of rDNA ITS( internal transcribed spacer) sequences analysis,and MEGA software. The results showed that there was significant relation between the mycorrhizal fungus of C. yunnanense and that of C. guttatum,in terms of specificity. In another word,mycorrhizal fungus has a obvious tendency in symbiosis with specific Cypripedium. The sequences of mycorrhizal fungus were uploaded to NCBI,and the access numbers were lcl51879,lcl25153,lcl48033,lcl38377,lcl14203,lcl24557,lcl52287 and lcl46937.