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Position: Home > Articles > Purification and properties of xylanase from Anoplophora chinensis (Frster) Acta Entomologica Sinica 2002,45 (2) 165-169

星天牛幼虫肠道木聚糖酶的纯化和性质

作  者:
董亚敏;殷幼平;曹月青;何正波
单  位:
重庆大学生物工程学院
关键词:
星天牛;木聚糖酶;纯化;性质
摘  要:
星天牛Anoplophorachinensis (F rster)幼虫肠道匀浆液经 80 %丙酮沉淀、Q Sepharose阴离子交换柱层析、PAGE制备电泳等方法纯化后 ,获得在SDS PAGE上呈现单一区带的木聚糖酶。该酶的分子量约 2 5kD ,等电点约 4 0 ,最适温度 5 0℃ ,最适pH 5 4,pH 3 0~ 7 8对酶活性的恢复无大的影响 ,5 0℃保温 2h仍有 6 0 %酶活性。Hg2 +、MnO-4 、变性剂SDS完全抑制该酶活性 ,Cu2 +、Mn2 +、Ag+、Zn2 +、Pb+、脲对酶活性有强烈的抑制作用。该酶具有水解纤维素的交叉活性 ,其Km 值为2 47mg mL ,Vmax为 0 6IU mL。
译  名:
Purification and properties of xylanase from Anoplophora chinensis (Frster)
作  者:
DONG Ya Min, YIN You Ping *, CAO Yue Qing, HE Zheng Bo (School of Bioengineering, Chongqing University, Chongqing 400044, China)
关键词:
Anoplophora chinensis (Frster); xylanase; purification; properties
摘  要:
A xylanase was purified from the homogenate of alimentary canal of Anoplophora chinensis (Frster) by acetone precipitation, Q Sepharose ion exchange chromatography and preparative PAGE. The xylanase had a molecular mass approximate 25 kD and pI 4 0 For the action of the enzyme isolated, the most optimal temperature and pH were 50℃ and 5 4 respectively. About 60% of the enzyme activity remained after incubating it at 50℃ for 2 hours. The enzyme could be inhabited by Hg 2+ ,MnO - 4,SDS,Cu 2+ ,Mn 2+ ,Ag +,Zn 2+ ,Pb + and Urea. It could hydrolyse carboxymethylcellulose (CMC). The enzyme had a K m of 2 47 mg/mL and a V max of 0 6 IU/mL for birchwood xylan at 50℃ and pH 4 4

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