关键词：
分子核型;染色体;荧光原位杂交;荧光分带;重复序列
摘 要：
近年来,荧光原位杂交及荧光分带等细胞遗传学技术的发展,提供了更多的染色体信息,使科研工作者能从更精细水平上对植物进行核型分析,更好地分辨基因组内各条非同源染色体,以及进行分化程度小的基因组间的比较。这种核型分析技术为研究染色体行为和变异,单个基因及转基因在基因组内的定位等开辟了新的途径。现在阐述以多色荧光原位杂交(mFISH)、基因组原位杂交(GISH)、染色体绘染(chromosome painting,CP)等荧光原位杂交为基础的染色体分子核型基础上,分析了荧光分带技术(fluorochrome banding)在分子核型中的应用,并对染色体分子核型技术在植物基因组功能研究领域的应用进行了展望。
译 名：
Research Advances in Plant Chromosomal Molecular Karyotyping
作 者：
ZHANG Li-peng;LIU Bo;CHEN Cheng-bin;College of Life Science,Nankai University;
关键词：
molecular karyotype;;chromosome;;fluorescence in situ hybridization(FISH);;fluorochrome banding;;repetitive sequences
摘 要：
Recent developments on multiple cytogenetic techniques such as fluorescence in situ hybridization(FISH)and fluorochrome banding,provide us more information on chromosomes,which enable detailed karyotype analysis of plants. As chromosome marks,signals showed by multicolor FISH using different repetitive sequences as probes and/or fluorochrome bands,improves our ability to distinguish each of the nonhomologous chromosomes within a genome,and to distinguish slightly diverged genomes from each other.Such karyotype analysis opens new avenues for the study of chromosomal variation and behavior,as well as for the localization of individual genes and transgenes to genomic position. In this paper,the multicolor fluorescence in situ hybridization(mFISH),genomic in situ hybridization(GISH), chromosome painting dye(chromosome painting,CP),such as fluorescence in situ hybridization-based molecular chromosome karyotype were described,based on the analysis of the fluorescence banding techniques(fluorochrome banding)applications in molecular karyotype and molecular karyotype and chromosome technology in the field of plant genomics functional studies were discussed.
