Position: Home > Articles > Cloning and Sequence Analysis of GIF Gene of Contagious Ecthyma Virus Inner Mongolia Isolate(OV/nm-05)
Animal Husbandry and Feed Science
2012,33
(4)
17-19
羊传染性脓疱病毒内蒙古分离株(OV/nm-05)GIF基因的克隆及序列分析
作 者：
Guan Ping-yua
单 位：
Xininige
关键词：
ov;05;95;gif gene;nm;inner mongoli
摘 要：
参考GenBank中羊传染性脓疱NZ2毒株GIF(GM-CSFinhibitory factor)基因序列,设计合成特异性引物,以羊传染性脓疱病毒内蒙古分离毒株(OV/nm-05)为模板,提取总DNA,用PCR技术特异扩增出该毒株GIF基因片段,将其克隆到pEASY-T1载体,鉴定后测序。应用计算机软件将OV/nm-05基因测序结果与参考毒株OV-NZ2、OV-D1701、OV-IA82、OV-SA00、OV-F07.808R、OV-MUK59/05、OV-F94.848R的GIF基因序列进行比较。结果表明,OV/nm-05与7株参考毒株的同源性分别为95.7%、98.2%、96.7%、95.5%、95.9%、95.5%、95.1%。
译 名：
Cloning and Sequence Analysis of GIF Gene of Contagious Ecthyma Virus Inner Mongolia Isolate(OV/nm-05)
作 者：
LU Yan,SHEN Zhi-yi,GUAN Ping-yuan,Xininigen(College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China)
关键词：
contagious ecthyma virus;PCR;cloning;sequence analysis
摘 要：
A pair specific primer were designed and synthesized according to the published GIF(GM-CSF inhibitory factor) sequence of NZ2 strain in GenBank.Total DNA of the contagious ecthyma virus(CEV) strain(OV/nm-05) which was isolated in Inner Mongolia was extracted,and the GIF gene was amplificated.The GIF gene was cloned into pEASY-T1 plasmids and then sequenced.The acquired GIF gene sequences of OV/nm-05 was compared with those of 7 reference strains like OV-NZ2,OV-D1701,OV-IA82,OV-SA00,OV-F07.808R,OV-MUK59/05 and OV-F94.848R.Results showed that the GIF gene homology of OV/nm-05 with 7 reference strains was 95.7%,98.2%,96.7%,95.5%,95.9%,95.5% and 95.1%,respectively.
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