当前位置: 首页 > 文章 > 用AFLP标记鉴定带有簇毛麦抗白粉病基因的小麦易位系 华北农学报 2000,15 (4) 29-34
Position: Home > Articles > Identification of Haynaldia villosa Translocation Lines with Resistant Gene to Powdery Mildew in Wheat by AFLP Analysis Acta Agriculturae Boreali-Sinica 2000,15 (4) 29-34

用AFLP标记鉴定带有簇毛麦抗白粉病基因的小麦易位系

作  者:
白建荣;刘润堂;郭秀荣;侯变英;PeterLangridge
单  位:
山西省农业科学院作物遗传研究所;澳大利亚国家研究委员会基础和应用分子生物学特别中心
关键词:
易位;AFLP;小簇麦;簇毛麦
摘  要:
对 4个小簇麦及小麦亲本和抗源供体簇毛麦进行了AFLP分析 ,确定了 4个小簇麦是均含有一段簇毛麦DNA的易位系。从得到的 3个与该基因可能较紧密连锁的标记和 7个不太紧密连锁的标记中 ,推测 4个易位系中簇毛麦DNA的长短不一样。文中还讨论了AFLP作为一种准确、快速鉴定易位系方法的可行性
译  名:
Identification of Haynaldia villosa Translocation Lines with Resistant Gene to Powdery Mildew in Wheat by AFLP Analysis
作  者:
BAI Jian rong 1,LIU Run tang 1,GUO Xiu rong 1,HOU Bian ying 1, Peter Langridge 2 (1 Crop Genetics Institute,Shanxi Academy of Agricultural Sciencs,Taiyuan Shanxi 030031,China; 2 ARC Special Research Centre for Basic and Applied Plant Molecula
关键词:
Translocation;AFLP;Wheat Haynalidia villosa;Haynalidia villosa
摘  要:
To identify the resistance for powdery mildew from Haynaldia villosa in 4 wheat Haynaldia villosa lines,we analysed them and their parents(common wheat,Taiyuan 768 and Haynaldia villosa) by AFLP method.The results suggested that 4 wheat Haynaldia villosa lines were all translocations and their resistant genes come from Haynaldia villosa.From 3 markers probably close linked to the resistant gene and 7 markers it is infered that the lengths of DNAs from Haynaldia villosa are different.AFLP is a reliable and efficient method for the identification of translocation.

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