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大豆疫霉菌部分生物学特性及其药剂筛选研究

作  者:
兰成忠;陈庆河;赵健;邱荣洲;李本金;翁启勇
单  位:
福建省农业科学院植物保护研究所
关键词:
大豆;大豆疫霉菌;生物学特性;化学防治
摘  要:
采用大豆疫霉菌在不同条件下菌丝生长速度法研究了大豆疫霉菌的部分生物学特性,并应用杀菌剂室内生测、盆栽药剂防效对药效作了评价。研究结果表明,大豆疫霉菌营养生长的最适温度为25~30℃;最适pH为6;光暗交替有利于该菌营养体的生长,在Rye或CA培养基上生长最快。室内药效测定结果表明,烯酰吗啉EC50为0.165 4μg/mL,抑菌效果最好,甲霜灵、甲霜灵.锰锌和氟吗啉.锰锌的EC50分别为0.261 00、.451 0和0.984 2μg/mL,效果次之。盆栽试验结果表明,几种药剂在活体条件下对大豆疫病的防治效果较好,并有较长持效期。
译  名:
Biological characteristics and screening of fungicides against Phytophthora sojae on soybean
作  者:
Lan Chengzhong,Chen Qinghe,Zhao Jian,Qiu Rongzhou,Li Benjin,Weng Qiyong(Institute of Plant Protection,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China)
关键词:
soybean;Phytophthora sojae;biological characteristics;chemical control
摘  要:
To study the biological characteristics of Phytophthora sojae and screen effective fungicides against soybean blight,mycelial growth rate of P.sojae was tested under different conditions and some fungicides were screened in the laboratory and greenhouses.The results showed that the optimum temperature for mycelial growth of P.sojae ranged from 25 ℃ to 30 ℃,and the optimum pH was 6.Mycelia grew well in rye and carrot agar media.Light was needed for the growth of mycelia,and mycelia grew well with a 12 h light period per day.In vitro experiments showed that dimethomorp,metalaxyl,metalaxyl-M mancozeb and flumorph effectively inhibited the growth of P.sojae.The EC50 values of dimethomorp,metalaxyl,metalaxyl-M mancozeb and flumorph were 0.165 4 μg/mL,0.261 0 μg/mL,0.451 0 μg/mL and 0.984 2 μg/mL,respectively.Dimethomorp had the lowest EC50 value and exhibited the highest inhibitory action to P.sojae.Greenhouse tests showed that flumorph,dimethomorph and metalaxyl were effective in controlling Phytophthora damping off and root rot of soybean in vivo and the inhibition lasted for a longer period of time.All the data showed that flumorph,dimethomorph and metalaxyl could be used to control Phytophthora damping off and root rot of soybean in fields.

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