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Position: Home > Articles > Comparison of three methods of measuring rhamnolipids using sugar development process Journal of Henan Institute of Science and Technology(Natural Science Edition) 2012,40 (5) 1-4

基于糖显色法测定鼠李糖含量的方法研究

作  者:
张祥胜;张玉辉;戴翠娥;许德军
单  位:
河南科技学院;盐城师范学院生命科学与技术学院
关键词:
鼠李糖脂;苯酚-硫酸法;苔黑酚-硫酸法;蒽酮-硫酸法;含量测定
摘  要:
为寻求快速而简单的测定铜绿假单胞菌产生的鼠李糖脂含量的方法,采用苯酚-硫酸法、苔黑酚-硫酸法、蒽酮-硫酸法3种比色法分别测定鼠李糖脂,并绘制鼠李糖脂-吸光度标准曲线,通过进一步的含量测定比较其精确度.结果表明:蒽酮-硫酸法准确度较高,方法更简便可行.
译  名:
Comparison of three methods of measuring rhamnolipids using sugar development process
作  者:
Zhang Xiangsheng1,Zhang Yuhui2,Dai Cuie1,Xu Dejun1(1.College of Life Science and Technology,Yancheng Teachers University,Yancheng 224002,China; 2.Henan Institute of Science and Technology,Xinxiang 453003,China)
关键词:
rhamnolipid;phenol-sulfuric acid method;orcinol-sulfuric acid method;anthrone sulfuric acid method;determination methods
摘  要:
Phenol-sulfuric acid method,orcinol-sulfuric acid method and anthrone-sulfuric acid method were employed to determine the levels of rhamnolipids and to obtain the standard rhamnolipid concentration-absorbance standard curves,and further recovery experiments to compare the accuracy of three kinds of methods.The results show that anthrone-sulfuric acid method got the greatest accuracy and reliability.

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