Position: Home > Articles > Inhibition to advanced glycation end- products and activity evaluation of sap of banana stem
Science and Technology of Food Industry
2016,37
(3)
49-53+57
香蕉茎秆汁液抑制晚期糖基化终末产物活性评价
作 者：
商文婷;盛占武;谷满屯;郑丽丽;艾斌凌
单 位：
中国热带农业科学院海口实验站/海南省香蕉遗传改良重点实验室;中国热带农业科学院海口实验站;海南大学食品学院中国热带农业科学院海口实验站海南省香蕉遗传改良重点实验室海口市香蕉生物学重点实验室
关键词：
香蕉茎秆汁液;α-葡萄糖苷酶;α-淀粉酶;晚期糖基化终末产物
摘 要：
为研究香蕉茎秆汁液抑制晚期糖基化终末产物(AGEs)活性,选用α-葡萄糖苷酶和α-淀粉酶抑制模型研究其对碳水化合物代谢关键酶的抑制作用,并采用Lineweaver-Burk双倒数法研究其动力学性质。同时对AGEs的抑制和清除ABTS~+·的能力也进行了评价。结果表明,香蕉茎秆汁液对α-葡萄糖苷酶的半抑制浓度(IC_(50))为1.51 mg/m L,对α-葡萄糖苷酶的抑制类型为混合型抑制。香蕉茎秆汁液对α-淀粉酶的半抑制浓度(IC_(50))是8.64 mg/m L,抑制类型为不可逆抑制。通过实验研究,香蕉茎秆汁液具有一定清除ABTS~+·的能力,对牛血清白蛋白果糖模型AGEs也具有一定的抑制作用。香蕉茎秆汁液可抑制体外AGEs的生成。
译 名：
Inhibition to advanced glycation end- products and activity evaluation of sap of banana stem
作 者：
SHANG Wen- ting;SHENG Zhan-wu;GU Man-tun;ZHENG Li-li;AI Bin-ling;YANG jin-song;College of Food Science,Hainan University;Haikou Experimental Station,Chinese Academy of Tropical Agricultural Sciences;Hainan Key Laboratory of Banana Genetic Improvement,Chinese Academy of Tropical Agricultural Sciences;
关键词：
sap of banana stem;;α-glucosidase;;α-amylase;;advanced glycation end-products
摘 要：
To study the inhibition to advanced glycation end- products( AGEs) activity by sap of banana stem,the inhibition of key enzymes in carbohydrate metabolism was evaluated by using α- glucosidase and α- amylase inhibition model.Inhibitory kinetic parameters were determined by Lineweaver- Burk plot.Meanwhile,the abilities of inhibiting AGEs and removing ABTS~+· were also evaluated.The results showed that the median inhibitory concentration( IC_(50)) of sap of banana stem on α-glucosidase was 1.51 mg /m L and the type of inhibition was mixcompetitive inhibition. The median inhibitory concentration( IC_(50)) of sap of banana stem on α- amylase was8.64 mg / m L and the type of inhibition was irreversible inhibition. Through experiment,sap of banana stem had a scavenging activity on removing ABTS~+·also had a certain capacity of inhibiting AGEs.The sap of banana stem had a certain inhibitory activity against AGEs.
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