Effect of the down-regulation of cofilin CFL2 on the key factors in the signal pathway of muscle fiber in mouse myoblast C2C12

HAN Jiaqi;SU Yuhong;XIE Fang;TIAN Yumin;ZHU Hongyan;College of Animal husbandry and Veterinary,Jinzhou Medical University;The Key Laboratory of Quality and Safety Engineering of Animal Products of Liaoning Province;

cofilin;;CFL2;;down-regulation;;C2C12 cell;;muscle fiber;;signal pathway

To investigate the effect of cofilin CFL2 low expression on the key factors in signal pathway of muscle fiber,mouse myoblast C2C12 was transfected by shRNA recombinant vectors targeting CFL2 gene according to RNAi method.The mRNA and protein expression levels of CFL2 and the key factors of signal pathway(MEF2 C,sk MLCK,Rho A,AMPKα2,p38 and Ca M) in the low expression groups were detected by Real-time PCR and Western blot.Results showed that in the low expression groups,the mRNA levels of key factors in signal pathway such as MEF2 C,Ca M,P38 and AMPKα2 were down-regulated and sk MLCK was increased significantly(P<0.01).The protein levels for key factors MEF2 C and Ca M in three transfected cell strains were down-regulated significantly(P<0.05 or P<0.01).The other four factors(p38,Rho A,AMPKα2 and sk MLCK) showed no significant difference(P > 0.05).It suggests that CFL2 gene silencing caused the low expression of MEF2 C and Ca M.There was a significantly positive or negative correlation between CFL2 and Ca M or sk MLCK.