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当前位置: 首页 > 文章 > 春雷霉素在番茄幼苗中的吸收与转运机制 现代农药 2022,21 (1) 47-52
Position: Home > Articles > Mechanism Underlying Absorption and Transportation of Kasugamycin in Tomato Seedlings Modern Agrochemicals 2022,21 (1) 47-52

春雷霉素在番茄幼苗中的吸收与转运机制

作  者：
张洪珍;卫国羽;赵微;胡安龙
单  位：
贵州大学作物保护研究所;贵州大学农学院
关键词：
春雷霉素;番茄;双向运输;能量抑制剂;单糖转运蛋白
摘  要：
为明确春雷霉素在植物体内的吸收传导特性,向番茄幼苗根部或叶部施药,采用高效液相色谱串联质谱法分别测定了春雷霉素在根、茎、叶中的积累量;其次探究了能量抑制剂二硝基苯酚(DNP)、羰基氰化物间氯苯腙(CCCP)、单糖转运蛋白竞争性底物(根皮苷、D-葡萄糖)和在不同pH及温度处理下番茄幼苗对春雷霉素的吸收影响。结果表明,150 mg/L春雷霉素处理番茄幼苗根部3 h后,在根部和茎部检测到的春雷霉素含量分别为172.57、12.71μg/L,6 h后药剂可到达植株叶部;500 mg/L春雷霉素浸施幼苗中部叶片24 h后,在根、茎上部叶均能检测到目标药剂;不同浓度的能量抑制剂和单糖转运蛋白竞争底物对春雷霉素的吸收有显著的抑制作用,且吸收对温度和pH较敏感。综上所述,在番茄体内,单糖转运蛋白协助主动运输春雷霉素,且具有双向传导特性。该研究为后期双向输导型农药的开发和田间科学合理施药提供依据。
译  名：
Mechanism Underlying Absorption and Transportation of Kasugamycin in Tomato Seedlings
作  者：
ZHANG Hongzhen;WEI Guoyu;ZHAO Wei;HU Anlong;Institute of Crop Protection, Guizhou University;College of Agriculture, Guizhou University;
关键词：
kasugamycin;;tomato;;bidirectional transport;;energy inhibitor;;monosaccharide transport protein
摘  要：
In order to clarify the absorption and conduction property of kasugamycin in plants, the kasugamycin was applied to the roots or leaves of tomato seedlings. The accumulations of kasugamycin in roots, stems and leaves were determined by HPLC-MS/MS. Furthermore, the energy inhibitors dinitrophenol(DNP), carbonyl cyanide meta-chlorophenylhydrazone(CCCP), monosaccharide transport protein competitive substrate(phlorizin, D-glucose), and changes in absorption under different pH and temperature treatments were explored. The results showed that after treatment with 150 mg/L kasugamycin on the roots of tomato seedlings for 3 h, the contents of kasugamycin in the roots and stems were172.57 and 12.71 μg/L, respectively, followed by reaching the leaves of the plants after 6 h. After spraying 500 mg/L kasugamycin on the middle leaves of the seedlings for 24 h, the target agentia could be detected in the roots and upper leaves of the stems. Different concentrations of energy inhibitors and competitive substrates had significant inhibitory effects on the absorption of kasugamycin in tomato seedlings, and absorption of kasugamycin were sensitive to temperature and pH. In summary, monosaccharide transport protein had property of bidirctional conduction in tomato seedlings and assisted an active transport process for kasugamycin. The study provided a basis for the further development of pesticides bidirectional transport and scientific and rational application of pesticides in the field.

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