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Position: Home > Articles > Study on Determination of Kojic Acid in Food by High Performance Liquid Chromatography FOOD SCIENCE 2005,26 (7) 205-207

食品中曲酸的色谱分析

作  者:
聂西度;谢华林;谭文莉;李爱阳
单  位:
湖南工学院化工系;中南大学化学化工学院应化系
关键词:
高效液相色谱法;曲酸;食品;四丁基溴化胺
摘  要:
建立了食品中曲酸(KA)的离子对高效液相色谱法。采用0.01mol/L磷酸氢二钠-甲醇(95:5,V/V)为流动相,添加1.0mmol/L四丁基溴化胺(TBA),检测波长226nm,样品用40%甲醇超声提取,曲酸在Nova-PakC18柱上获得了良好分离。该方法的检出限为0.032mg/L,回收率为95.7%~103.2%,相对标准偏差为1.16%~2.01%。结果表明,方法的检出限为0.032mg/L,回收率为95.7%~103.2%,相对标准偏差为1.16%~2.01%。该法准确、快速、简便,应用于食品中曲酸的测定,结果满意。
译  名:
Study on Determination of Kojic Acid in Food by High Performance Liquid Chromatography
作  者:
NIE Xi-du1,XIE Hua-lin1,*,TAN Wen-li1,LI Ai-yang2(1.Department of Chemical Engineering,Hunan Institute of Technology, Hengyang 421008,China;2.College of Chemistry and Chemical Engineering,Central South University,Changsha 410083, China)
关键词:
high performance liquid chromatography;kojic acid;food;tetrabutylammonium bromide
摘  要:
In this paper a new and an efficient method for analyzing kojic acid(KA) in food by ion-pair high performance liquidchromatography was developed.Nova-PakC18 column was used and mobile phase was 0.01mol/L Na2HPO4-methanol(95:5,V/V)with addition of 1.0mmol/L tetrabutylammonium bromide(TBA).The wavelength 226nm was selected for detection.Sampleswere extracted with 40%methanol by ultrasonic method. Under the optinum conditions, the detection limit was 0.032mg/L. Therecoveries were 95.7%~103.2% and the relative standard deviations were 1.16%~2.01%. This method was accurate, quick-acquired and convenient.It is used in the determination of kojic acid with satisfactory result.

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