中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 云南省南苜蓿和天蓝苜蓿根瘤菌BOX-PCR分析 安徽农业科学 2009,37 (12) 77-79
Position: Home > Articles > Study on the BOX-PCR Analysis of Rhizobium in the Bur Clover and Black Medic in Yunnan Province Journal of Anhui Agricultural Sciences 2009,37 (12) 77-79

云南省南苜蓿和天蓝苜蓿根瘤菌BOX-PCR分析

作  者：
张斌;李乔仙;刘晓云
单  位：
河北大学生命科学学院;西南林学院资源学院;云南省草地动物科学研究院
关键词：
根瘤菌;BOX-PCR;苜蓿;南苜蓿;天蓝苜蓿
摘  要：
[目的]进行南苜蓿和天蓝苜蓿根瘤菌的BOX-PCR分析,为苜蓿高效固氮根瘤菌的筛选和接种剂的研究提供理论依据。[方法]采用boxA1R单引物对90株根瘤菌供试菌株及5株标准菌株进行BOX-PCR分析,并进行聚类分析。[结果]经过扩增得到了较多的条带,90株供试菌株共有31种BOX图谱类型。聚类分析结果显示,所有菌株在40%相似性水平上聚为一群;而在69%的相似性水平上,供试菌株可细分为7个遗传群,群1的菌株全部分离自云南省江川县路居镇天蓝苜蓿的根瘤,群3内的菌株都分离自德宏州盈江县的南苜蓿根瘤,并与标准菌株Enisfer melilotiUSDA1002T聚在一群,在群4、5、6中,除SWF67340的宿主为南苜蓿外,其他菌株的宿主都为天蓝苜蓿,群7中,除SWF66320、SWF67343、SWF65116和SWF673704株菌的宿主为天蓝苜蓿外,其余菌株的宿主都为南苜蓿。[结论]供试菌株具有丰富的遗传多样性,BOX-PCR能够区分它们之间的差异;大多数菌株按宿主及采集地聚群,但也有交叉。
译  名：
Study on the BOX-PCR Analysis of Rhizobium in the Bur Clover and Black Medic in Yunnan Province
作  者：
ZHANG Bin et al(College of Resource,Southwest Forestry College,Kunming,Yunnan 650224)
关键词：
Rhizobium;BOX-PCR;Alfalfa;Bur clover;Black medic
摘  要：
[Objective] The theoretical basis of the research on the screening and inoculants of high-efficient nitrogen-fixing alfalfa rhizobium was provided through the BOX-PCR analysis of bur clover and black medic.[Method] 90 experimental rhizobium strains and five standard rhizobium strains were tested with boxA1R single primer by means of BOX-PCR analysis and cluster analysis.[Results] More bands were produced after the strains were amplified and there were 31 kinds of BOX type mappings in 90 experimental rhizobium strains.The results from cluster analysis showed that at the similarity level of 40%,all strains could be in a group,and at the similarity level of 69%,the strains could be divided into seven genetic groups.All of the strains in Group 1 were isolated from the black medic rhizobium from Luju township of Jiangchuan County;all of the strains in Group 3 were isolated from the bur clover rhizobium from Yingjiang County of Dehong State in Yunnan Province,which were clustered in same group as standard rhizobium strain-Enisfer meliloti USDA 1002T;the strains in Group 4,Group 5 and Group 6,except the strain-SWF67340,were isolated from the bur clover rhizobium;4 strains in Group 7,with the exception of SWF66320,SWF67343,SWF65116 and SWF67370,were isolated from the black medic rhizobium,and the other strains,from bur clover rhizobium.[Conclusion] The experimental strains had a wealth of genetic diversity.BOX-PCR analysis could distinguish the differences between them.The majority of strains were clustered by the host and collected location,but there was also cross among the strains.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46