中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 茶树牻牛儿基牻牛儿基焦磷酸合成酶基因CsGGDPS的克隆及表达分析 茶叶科学 2017,37 (1) 86-96
Position: Home > Articles > Cloning and Expression Analysis of Geranylgeranyl Diphosphate Synthase Gene CsGGDPS in Tea Plant(Camellia sinensis) Journal of Tea Science 2017,37 (1) 86-96

茶树牻牛儿基牻牛儿基焦磷酸合成酶基因CsGGDPS的克隆及表达分析

作  者：
姚雪倩;岳川;杨国一;陈丹;张冬桃;陈桂信;叶乃兴
单  位：
福建农林大学园艺学院中国乌龙茶协同创新中心
关键词：
茶树;牻牛儿基牻牛儿基焦磷酸合成酶;茶叶香气;基因表达
摘  要：
以铁观音芽叶为材料,验证从茶树转录组数据库中筛选到的1条牻牛儿基牻牛儿基焦磷酸合成酶(GGDPS)的编码全长序列c DNA。该c DNA全长1 661 bp,含有1个1 137 bp完整的开放阅读框,命名为Cs GGDPS。该基因编码378个氨基酸,氨基酸序列具有类异戊二烯合成酶家族的5个保守域和2个特征功能域;序列分析显示,该c DNA与其他植物GGDPS高度保守,与三七(Panax notoginseng)的亲缘关系最近。Cs GGDPS属于不稳定、亲水蛋白,可能定位到叶绿体中,不存在跨膜结构,无信号肽,发生磷酸化的位点可能有20个;二级结构主要由α-螺旋构成,三级结构与拟南芥GGPPS11匹配度最高。实时荧光定量PCR结果表明,在茶树发育过程和不同叶位Cs GGDPS的表达量随芽叶成熟度的增加呈上升趋势,随着做青过程的进行,Cs GGDPS的表达量逐渐升高;Cs GGDPS在父本黄旦、母本铁观音和子一代金观音中均有表达,但表达量存在差异。
译  名：
Cloning and Expression Analysis of Geranylgeranyl Diphosphate Synthase Gene CsGGDPS in Tea Plant(Camellia sinensis)
作  者：
YAO Xueqian;YUE Chuan;YANG Guoyi;CHEN Dan;ZHANG Dongtao;CHEN Guixin;YE Naixing;College of Horticulture, Fujian Agriculture and Forestry University, Collaborative Innovation Center of Chinese Oolong Tea Industry;
关键词：
Camellia sinensis;;GGDPS;;tea aroma;;gene expression
摘  要：
A full-length c DNA sequence encoding geranylgeranyl diphosphate synthase( GGDPS) was isolated from transcriptome database of tea plant, cloned from C. sinensis cv. Tieguanyin and named as CsGGDPS. The c DNA length of CsGGDPS was 1 661 bp, with an open reading frame(ORF) of 1 137 bp and deduced protein of 378 amino acids. The protein was deduced to contain 5 conserved domains with 2 funct ional domains of Isoprenoid-Biosyn-C1 superfamily. The sequence analysis showed that CsGGDPS was highly conserved and had the closest genetic relationship with Panax notoginseng. CsGGDPS was an instability and hydrophilic protein, which was predicted to be located in chloroplast but with no transmembrane structure and signal peptide. There were 20 phosphorylation sites within the polypeptide chain. Alpha helix was predicted to be the major secondary structure of CsGGDPS. The three-dimension structure of CsGGDPS was highly similar to GGPPS11 from Arabidopsis thaliana. The quantitative real-time PCR showed that CsGGDPS expression was increased during the developmental process and increased with the age of tea leaves. Meanwhile, its expression was also enhanc ed during the Zuoqing procedure. CsGGDPS was ubiquitously expressed in the C. sinensis cv. Huangdan, cv. Tieguanyin and their first filial generation cv. Jinguanyin, but with different expression levels.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46