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农业环境科学学报
2013,32
(01)
153-159
Position: Home > Articles > Isolation and Culture of Thiobacillus denitrificans from Different Area of the Intensive Pond System and Their Capacities of Removing Nitrate
Journal of Agro-Environment Science
2013,32
(01)
153-159
养殖池塘系统脱氮硫杆菌(Thiobacillus denitrificans)的分离、生长特性及脱氮特征研究
作 者：
范立民;裘丽萍;陈家长;宋超;胡庚东;瞿建宏;孟顺龙;吴伟
单 位：
农业部长江下游渔业生态环境监测中心;中国水产科学研究院内陆生态环境和资源重点开放实验室;中国水产科学研究院淡水渔业研究中心
关键词：
养殖池塘系统;脱氮硫杆菌;生长特性;脱氮效能
摘 要：
分别采集集约化养殖吉富罗非鱼(Oreochromis niloticus)的池塘底泥、表层水、塘边裸露土壤样品,采用脱氮硫杆菌选择培养基富集培养、分离得到3株细菌,通过菌落特征、形态学特征、生理生化检验和16S rDNA序列分析等手段,鉴定为脱氮硫杆菌(Thiobacillus denitrificans),命名为TD1、TD2和TD3。在30℃下,采用静置、厌氧培养实验研究这3株脱氮硫杆菌的生长繁殖特征、对硝酸盐氮的去除速率特征、硫酸根产生状况、亚硝酸盐产生情况、培养液pH值和氧化还原电位变化特征等方面的差异进行研究,探索养殖环境修复中应用的可能性。结果表明:在一定的时间内菌株生长速率、介质pH降低速率和氧化还原电位上升幅度均表现为TD1>TD2>TD3;菌株硝态氮脱除效率和硫酸根浓度升高速率均表现为TD1>TD2>TD3,亚硝酸盐产生情况则表现为TD1
译 名：
Isolation and Culture of Thiobacillus denitrificans from Different Area of the Intensive Pond System and Their Capacities of Removing Nitrate
作 者：
FAN Li-min,QIU Li-ping,CHEN Jia-zhang,SONG Chao,HU Geng-dong,QU Jian-hong,MENG Shun-long,WU Wei*(Freshwater Fisheries Research Centre,Chinese Academy of Fishery Sciences,Fishery Eco-Environment Monitoring Center of Lower Reaches of Yangtze River,Ministry of Agriculture,Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries,Wuxi 214081,China)
关键词：
aquaculture pond system;Thiobacillus denitrificans;growth characteristics;denitrifying effect
摘 要：
In this study,an intensive pond in which GIFT tilapia(Oreochromis niloticus) were cultured was used.And sampling were done in different areas of the pond system,including sediments of the pond,the waters 50 centimeters below the surface and the bare soil at the edge of the pond.After that,enrichment and elective cultures were prepared anaerobically on these samples in 30 centigrade by incubating glass-stoppered bottles with the medium for Thiobacillus denitrificans full filled.After the culture,by using the spread-plate method,three strains were got.Through the appearance of the colonies,the shape of the strains,the detection of physiological and biochemical indicators,and the analysis of the sequences of 16S rDNA,the strains was identified as Thiobacillus denitrificans,and they were named as TD1,TD2 and TD3.Through the studies on the characteristics of growth and reproduction,of the removing rates of the nitrate,of the production of the sulfate and nitrite,and of the change of the pH and redox potential in medium,the possibilities of being used in repairing of the aquaculture pond environment were determined to some extent.The results showed that:in most of the time of the experiment,the growth rates,the pH decreasing rates,and the redox potential increasing extent of the strains were all performed as TD1>TD2>TD3;and the trends of the nitrate removing rates and the increasing rates of the three strains were the same as above,however,the production of nitrite by the three strains showed different trends,which was TD1
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