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当前位置: 首页 > 文章 > 太行菊NAC转录因子基因OpNAC1的克隆及生物信息学分析 分子植物育种 2018 (11) 3477-3486
Position: Home > Articles > Cloning and Bioinformatics Analysis of a NAC Transcription Factor Gene OpNAC1 from Opisthopappus taihangensis Molecular Plant Breeding 2018 (11) 3477-3486

太行菊NAC转录因子基因OpNAC1的克隆及生物信息学分析

作  者：
梁芳;张燕;郝平安;崔波
单  位：
郑州师范学院生物工程研究所;河南农业大学生命科学学院
关键词：
太行菊;NAC;基因克隆;生物信息
摘  要：
NAC转录因子是植物中数量最大的转录因子,在植物的生长发育及各种逆境胁迫中发挥非常重要的作用。本研究以太行菊的叶片为研究材料,运用RT-PCR及RACE技术克隆得到一条太行菊的NAC基因完整的cDNA序列,命名为OpNAC1(GenBank登录号MF996370),并对其进行了生物信息学分析。分析表明,OpNAC1基因cDNA序列全长1 208 bp,ORF全长855 bp,编码284个氨基酸。预测其蛋白分子量32.68 kD,等电点6.46,属于不稳定亲水性蛋白。不含有信号肽和跨膜结构,很可能定位于细胞核。OpNAC1与菊花(ADQ20114.1)亲缘关系较近,同源性达93.7%。二级结构预测表明,OpNAC1蛋白由40个α-螺旋(14.08%)、47个β-折叠(16.55%)和197个无规则卷曲(69.37%)组成,与三级结构预测基本相符。研究结果为今后深入研究NAC转录因子在太行菊中的生物功能提供基础。
译  名：
Cloning and Bioinformatics Analysis of a NAC Transcription Factor Gene OpNAC1 from Opisthopappus taihangensis
作  者：
Liang Fang;Zhang Yan;Hao Ping'an;Cui Bo;Institute of Bioengineering, Zhengzhou Normal University;College of Life Sciences, Henan Agriculture University;
关键词：
Opisthopappus taihangensis;;NAC;;Gene cloning;;Bioinformatics
摘  要：
NAC transcri ption fa ctor is a transcription factor with the largest number in plant, and plays a very important role in plant growth and development and all kinds of stress response. In this study, the leaves of Opisthopappus taihangensis were used as materials. The complete cDNA sequence of NAC gene that named OpNAC1(GenBank accession No. MF996370) was obtained successfully through the cloning by RT-PCR and RACE method,which was then applied to bioinformatics analysis. The results indicated that the full-length of cDNA sequence of OpNAC1 gene was 1 208 bp, and the ORF was 855 bp with 284 amino acids encoded. Thus, OpNAC1 gene was predicted to be an unstable hydrophilic protein with 32.68 kD protein molecular weight and 6.46 isoelectric point,which had no signal peptide and transmembrane region, and was likely to locate in the nucleus. OpNAC1 gene was close to Chrysanthemum(ADQ20114.1) in genetic relationship with the homology of 93.7%. The secondary structure analysis indicated that OpNAC1 protein was composed of 40 α-helixs(14.8%), 47 β-sheets(16.55%) and 197 random coils(69.37%), which conformed to the prediction of the tertiary structure. The results might provide a basis for further research on the biological functions of NAC transcription factors in Opisthopappus taihangensis.

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