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当前位置: 首页 > 文章 > 干旱胁迫对马铃薯叶片内生细菌组成和结构的影响 华中农业大学学报 2024 (1) 79-88
Position: Home > Articles > Effect of drought stress on composition and structure of endosymbiotic bacteria in potato leaves Journal of Huazhong Agricultural University 2024 (1) 79-88

干旱胁迫对马铃薯叶片内生细菌组成和结构的影响

作  者：
吉泽;肖关丽;陈斌;李俊逸;姚遥;祝春月;杨金睿
单  位：
云南农业大学植物保护学院;云南农业大学农学与生物技术学院
关键词：
马铃薯;内生细菌;干旱胁迫;微生物群落;高通量测序
摘  要：
为解析马铃薯应对干旱胁迫的响应机制,采用Illumina Novaseq测序技术,对干旱胁迫下马铃薯叶片中的内生细菌16S rRNA进行高通量测序,研究干旱胁迫下马铃薯叶片内生细菌组成及多样性的变化.结果显示,在正常浇水和干旱胁迫下马铃薯叶片分别获得1548和2006条分类序列,其中正常浇水下特有OTU有287个,干旱处理下特有OTU有769个.在干旱胁迫下,内共生菌candidatus Portiera和杀雄菌属Arsenophonus的丰度较正常浇水下减低了95.9%和68.1%,拟杆菌门Muribaculaceae拟杆菌目Bacteroidales、毛螺菌属(Lachno?spira)、乳杆菌属(Lactobacillus)、毛螺菌科NK4A136群(Lachnospiraceae_NK4A136_group)、根瘤菌属(Rhizobi?um)、螺杆菌属(Helicobacter)、链球菌属(Streptococcus)、脱硫弧菌属(Desulfovibrio)在干旱处理较正常浇水下分别提高了203.8%、152.4%、81.8%、89.7%、285.6%、1428.0%、112.0%和148.4%;Shannon指数和Simpson指数均在干旱胁迫下显著高于正常浇水处理,ACE指数和Chao1指数在干旱胁迫和正常浇水下无显著差异.结果表明,干旱胁迫能影响马铃薯内生细菌群落结构组成,干旱胁迫下富集了内生优势菌门放线菌门和厚壁菌门,同时伯克霍尔德氏科细菌相对丰度增加,可能是马铃薯应对干旱胁迫作出的积极响应.
译  名：
Effect of drought stress on composition and structure of endosymbiotic bacteria in potato leaves
单  位：
College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China;College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China.
关键词：
potato%endosymbiotic bacteria%drought stress%microbial community%high throughput sequencing
摘  要：
High-throughput sequencing of 16S rRNA from endosymbiotic bacteria in potato leaves under drought stress was performed with Illumina Novaseq sequencing technology to understand the changes in the composition and structure of endosymbiotic bacteria in potatoes under drought stress.The effects of drought stress on the composition,structure,and diversity of endosymbiotic bacteria in potato leaves were studied.The results showed that 1548 and 2006 taxonomic sequences were obtained from potato leaves under normal watering and drought stress,respectively.Among them,287 were unique OTUs under normal watering and 769 were unique OTUs under drought stress.Under drought stress,the abundance of the endosymbiotic bacteria candidatus Portiera and Arsenophonus decreased by 95.9%and 68.1%compared with that under normal watering,while the abundance of Muribaculaceae,Lachnospira,Lactobacillus,Lachnospiraceae_NK4A136_group,Rhizobium,Helicobacter,Streptococcus and Desulfovibrio increased by 203.8%,152.4%,81.8%,89.7%,285.6%,1428.0%,112.0%and 148.4%respectively under drought stress compared with that under normal watering.The Shannon index and Simpson index under drought stress were significantly higher than that under normal watering,while the ACE index and Chao1 index under drought stress were not significantly different from that under normal watering.It is indicated that drought stress can affect the composition of endosymbiotic bacterial communities in potatoes.Under drought stress,the endosymbiotic dominant bacteria Actinomycetota and Bacillota were enriched,and the relative abundance of Burkholderiaceae bacteria increased,which may be the positive response of potato to drought stress.

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