Position: Home > Articles > Construction of Plant Expression Vector for the Simultaneous Silencing of FAD21- and FatBGenes in Cotton Seeds
Acta Agriculturae Boreali-occidentalis Sinica
2013,22
(10)
54-61
同步抑制棉籽FAD21-与FatB基因表达的RNAi载体构建
作 者：
赵彦朋;李艳军;孙杰;刘峰
单 位：
石河子大学农学院
关键词：
FAD21-基因;FatB基因;Gateway技术;双基因沉默;载体构建
摘 要：
采用PCR技术,分别扩增获得靶标基因FAD21-与FatB的功能区域片段426与501bp,通过PCR引物引入特定的酶切位点,采用DNA标准重组技术与Gateway技术相结合的方法,以表达载体pBI121及RNA干扰载体pANDA35HK为基础,利用种子特异性启动子,成功构建出棉籽特异抑制双基因表达的RNAi载体。
译 名：
Construction of Plant Expression Vector for the Simultaneous Silencing of FAD21- and FatBGenes in Cotton Seeds
作 者：
ZHAO Yanpeng;LI Yanjun;SUN Jie;LIU Feng;College of Agriculture,Shihezi University;
关键词：
FAD21-gene;;FatBgene;;Gateway technology;;Dual-Target gene silencing;;Vector construction
摘 要：
To regulate fatty acid metabolism of cottonseed by simultaneous inhibition of FAD21- and FatBgenes expression levels for improving the quality of cottonseed oil,the 426bp and 501bp fragments of Dual-Target gene FAD21- and FatB were respectively amplified by PCR technology in this study firstly.Combining DNA recombinant technique with Gateway technology,the cottonseed specific Dual-Target gene silencing RNAi vector was successfully constructed by introducing the seed specific promoter,based on the expression vector pBI121and RNAi vector pANDA35HK.This study provided the foundation for further to apply for cotton genetic transformation and improve the quality of cottonseed fatty acids.
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