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当前位置: 首页 > 文章 > 成都麻羊和波尔山羊生长激素基因HaeⅢ多态性的比较研究 黑龙江畜牧兽医 2005 (8) 13-14
Position: Home > Articles > Study on genetic polymorphism of Hae Ⅲ site of GH gene in Chengduma goat and beor goat Heilongjiang Animal Science and Veterinary Medicine 2005 (8) 13-14

成都麻羊和波尔山羊生长激素基因HaeⅢ多态性的比较研究

作  者：
白文林;王杰;尹荣焕;赵素君;罗光彬;姜午旗
单  位：
沈阳农业大学畜牧兽医学院;西南民族大学生命科学与技术学院;四川省畜牧科学研究院
关键词：
山羊;GH基因;PCR-RFLP
摘  要：
利用PCR-RFLP技术检测了成都麻羊和波尔山羊生长激素(G rowth Hormone,GH)基因的HaeⅢ酶切多态性。结果表明:成都麻羊和波尔山羊2个山羊群体中均存在GH基因HaeⅢ酶切位点的多态性,且均有A、B两个等位基因。成都麻羊群体等位基因A和B的基因频率分别为0.621 6和0.378 4;波尔山羊群体中等位基因A和B的基因频率分别为0.534 5和0.465 5。GH基因HaeⅢ酶切位点的基因型分布在两个山羊群体中均极显著偏离Hardy-W e inberg平衡定理(P<0.01),但两个群体间不存在显著差异(P>0.05)。
译  名：
Study on genetic polymorphism of Hae Ⅲ site of GH gene in Chengduma goat and beor goat
作  者：
BAI Wenlin~1,WANG Jie~2,YIN Ronghuan~1,et al(1.College of Animal Science and Veterinary, Shenyang Agricultural University,Shenyang 110161,China; 2. College of Life Science and Technology , Southwest Nationalities University , Chengdu 610041,China)
关键词：
Goats ; growth hormone gene ; PCR-RFLP
摘  要：
By the technology of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) , genetic polymorphism of HaeⅢ site of growth hormone gene in ChengduMa goats and Boer goats was tested. The result showed that the HaeⅢ sites controlled by allele A and B were polymorphic in Chengdu Ma goats and Boer goats. The allele frequencies of A and B were 0.621 6 and 0.378 4 in Chengdu Ma goats respectively; and the allele frequencies of A and B were 0.534 5 and 0.465 5 in Boer goats respectively. The genotype distribution of HaeⅢ sites in Chengdu Ma goats and Boer goats didn't fit significantly Hardy-Weinberg Law(P<0.01),but the difference of the genotype distribution between Chengdu Ma goats and Boer goats isn't significant(P>0.05).

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