Position: Home > Articles > Study on Isolation and Characterization of a DDT Degradation Bacterium Strain DH-7
Journal of Anhui Agricultural Sciences
2009,37
(13)
45-46+57
DDT降解菌DH-7的分离鉴定与降解特性研究
作 者：
李红权;马珦玻;李红梅;昌艳萍;赵盛;陈淑兰;周艳春
单 位：
河北大学基础医学院;河北大学生命科学学院
关键词：
铜绿假单胞球菌;DDT降解;16S rDNA
摘 要：
[目的]研究DDT降解菌的生物学、降解特性及其发酵条件的优化。[方法]从化工厂采集土样,分离、筛选到1株能够在好氧条件下DDT降解率较高的菌株DH-7,并对其进行研究。[结果]通过16S rDNA序列分析结合传统分类学方法初步确定菌株DH-7为铜绿假单胞菌。对菌体降解DDT特性的研究表明,该菌株对DDT降解10 d的降解率为73.6%。在优化培养条件后,该菌株10 d的降解率达81.4%。[结论]该研究结果为DDT污染土壤的生物修复提供依据。
译 名：
Study on Isolation and Characterization of a DDT Degradation Bacterium Strain DH-7
作 者：
LI Hong-quan et al (School of Basic Medical Science,Hebei University,Baoding,Hebei 071000)
关键词：
Pseudomonas aeruginosa;DDT degradation;16S rDNA
摘 要：
[Objective]The purpose of this research was to study biological characteristics,degradation characteristics and the optimization of fermentation conditions of DDT degradation bacterium.[Method] A bacterium strain DH-7 having capability of degrading DDT was isolated from the DDT contaminated soil of a chemical factory.The characteristics of DH-7 were studied.[Result]Based on the phenotype,physiological and biochemical characteristics,and the phylogenetic analysis of 16S rDNA sequence,the strain DH-7 was identified preliminarily as Pseudomonas aeruginosa.This strain could degrade DDT with degradation efficiency of 73.6% in 10 days.The degradation efficiency was up to 81.4% after the conditions of culture was optimized.[Conclusion]This study will provide the references for bioremediation of contaminated soil by DDT.
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