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二种槭树有效获得无菌试管苗的研究

作  者:
叶景丰;陈罡;马冬菁;潘文利;范俊岗;祁爽
单  位:
辽宁省生态公益林项目中心;辽宁省林业科学研究院
关键词:
槭树;外植体;消毒时间;培养基;培养条件
摘  要:
以挪威槭的成熟种子,自由人槭的顶芽、幼嫩茎段为外植体,研究了0.1%升汞不同消毒时间、不同培养条件、不同培养基对2种槭树有效获得无菌试管苗的影响。结果表明:挪威槭种子用0.1%升汞消毒8min,在MS+6-BA0.5mg/L+NAA0.2mg/L的培养基上,黑暗条件、温度24~26℃下,有效的获得了无菌试管苗;自由人槭以顶芽为外植体,0.1%升汞消毒6min,在MS+6-BA0.5(0.2)mg/L+NAA0.2mg/L培养基上,光照3000~5000lx、温度24~26℃下,同样有效的获得了无菌试管苗。
译  名:
Establishing Two Kinds of Maples Sterile Eullune System
作  者:
YE Jing-feng1,CHEN Gang1,MA Dong-jing1,PAN Wen-li1,FAN Jun-gang1,QI Shuang2(1.Liaoning Research Academy of Forestry Sciences,Shenyang,Liaoning 110032,China;2.Liaoning Ecological Commonweal Forest Centre of Project,Shenyang,Liaoning 110036,China)
关键词:
Maple;Explant;Sterilization time;Mediation;Culture condition
摘  要:
Explants from mature seeds of Acer platanoides Linnd,terminal bud and stem segments of Acer freemanii Autumn Blaze,key technology of establishing two kinds of maples sterile culture system was studied.The result showed that sterilized mature seeds of Acer platanoides Linnd with 0.1% mercuricchloride in 8 minutes,sterilized the best explant terminal bud of Acer freemanii Autumn Blaze with 0.1% mercuricchloride in 6 minutes,cultured two kinds of explants at the MS medium supplied with with 6-BA 0.5 mg/L,NAA 0.2 mg/L,the germfree young plant of test tube was effective obtained same.

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