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Position: Home > Articles > The Factors Affecting Genetic Transformation of Soybean Cotyledon Node Mediated by Agrobacterium tumefacions Biotechnology Bulletin 2015,31 (12) 91-96

农杆菌介导大豆子叶节影响因素的研究

作  者：
杨晓倩;李桂兰;刘晨光;董秋平;张锴;乔亚科
单  位：
河北科技师范学院生命科技学院
关键词：
农杆菌介导;大豆子叶节;共培养;单壁碳纳米管
摘  要：
为提高大豆遗传转化效率,采用农杆菌介导遗传转化的方法,以大豆子叶节为外植体,研究共培养阶段浸染液浓度及外界培养条件(共培养温度和天数)和培养基添加物对转化效率和芽诱导率的影响。结果显示,浸染液OD600=0.5的诱导率最高,共培养温度为24℃,共培养天数为10 d具有较高转化效率,共培养基中加入一定浓度的单壁碳纳米管(Single-wall carbon nanotube,NM)对诱导卡那霉素抗性不定芽有促进作用。PCR检测表明PHR1基因已整合到T1代大豆基因组中,初步证明可在大豆基因组中稳定遗传。
译  名：
The Factors Affecting Genetic Transformation of Soybean Cotyledon Node Mediated by Agrobacterium tumefacions
作  者：
Yang Xiaoqian;Li Guilan;Liu Chenguang;Dong Qiuping;Zhang Kai;Qiao Yake;Life Science and Technology Institute,Hebei Normal University of Technology;
关键词：
Agrobactium-mediated;;soybean cotyledon node;;co-culture;;single-wall carbon nanotube
摘  要：
It was to improve the efficiency of soybean genetic transformation, we used the soybean cotyledon node as explants via Agrobactium-mediated transformation method, and investigated the effect of bacterial concentration, co-culture temperature and time on the transformation efficiency and the rate of bud induction. The results showed that under the OD600 = 0.5 of infection solution concentration, and induction rate was the highest. The conversion efficiency was higher under the environment of co-culture temperature being 24℃ for 10 days. The single-wall carbon nanotubes added to culture medium improved the induction of the adventitious bud with kanamycin-resistance. Moreover, the PCR results indicated that PHR1 gene was integrated into T1 genome generation, preliminarily proving that the heredity of gene in the soybean genome was stable.

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