作 者：
严善春;程红;杨慧;袁红娥;张健;迟德富
关键词：
青杨脊虎天牛;植物挥发物;驱避;引诱;触角电位反应;行为反应;Y型嗅觉仪
摘 要：
测定了青杨脊虎天牛Xylotrechus rusticus(L.)雌、雄成虫对其寄主杨树中的水杨醛(0.95μmol/μL)和非寄主植物中0.3μmol/μL的叶绿醇、0.4μmol/μL的水芹烯和0.6μmol/μL的R型α-蒎烯、S型α-蒎烯、S型β-蒎烯、3-蒈烯、罗勒烯、香草烯和松节油等10种植物挥发性气味物质的触角电位(EAG)反应。结果表明,与对照相比,这10种植物挥发物多能引起成虫明显的EAG反应(P<0.05 ,P<0.01) ,其中雌虫对松节油、水杨醛、R型α-蒎烯和S型α-蒎烯的EAG反应较强;雄虫对R型α-蒎烯的EAG反应最强,松节油次之。根据雌虫对这10种挥发物EAG反应的强弱,进一步测定了雌虫对0.00006、0.0006、0.006、0.06、0.6、0.12μmol/μL的松节油、R型α-蒎烯、S型α-蒎烯以及0.000095、0.00095、0.0095、0.095、0.95、0.19μmol/μL的水杨醛的EAG和行为反应。结果表明,雌虫对松节油、水杨醛和R型α-蒎烯的EAG反应随气味物质浓度的增加而增加,水杨醛浓度增加到0.95μmol/μL、松节油和R型α-蒎烯浓度增加到0.6μmol/μL以后,EAG反应值趋于平稳;对S型α-蒎烯的反应随浓度的增加而呈线性增加。水杨醛浓度低于0.095时,对雌虫没有明显的定向作用(P>0.05) ,高于此浓度时表现为驱避作用(P<0.05) ;松节油在浓度低于或等于0.6μmol/μL时对雌虫表现为驱避作用,浓度为0.6时驱避效果最佳(P<0.01)。雌虫对R型α-蒎烯和S型α-蒎烯没有明显的定向行为反应。
译 名：
Effects of plant volatiles on the EAG response and behavior of the grey tiger longicorn,Xylotrechus rusticus (L.)(Coleoptera:Cerambycidae)
作 者：
YAN Shan-Chun, CHENG Hong, YANG Hui, YUAN Hong-E, ZHANG Jian, CHI De-Fu(College of Forestry, Northeast Forestry University, Harbin 150040, China)
关键词：
Xylotrechus rusticus; plant volatiles; repellent; lure; EAG response; behavior; Y-tube olfactometry
摘 要：
The electroantennogram (EAG) responses of female and male beetles of Xylotrechus rusticus (L.) to 10 volatiles were tested, including salicylaldehyde (0.95 μmol/μL) from poplar, the host plant, and phytol (0.3 μmol/μL), R(-)-α-phellandrene (0.4 μmol/μL), (1R)-(+)-α-pinene, (1S)-(-)-α-pinene, (1S)-(-)-β-pinene, (+)-3-carene, ocimene, β-myrcene and turpentine (0.6 μmol/μL) from non-host plant. The results showed that the 10 volatiles could evoke very significant EAG responses (P<0.01) of the adults compared with the control, paraffin. The females exhibited stronger EAG responses to turpentine, salicylaldehyde, (1R)-(+)-α-pinene, (1S)-(-)-α-pinene, while (1R)-(+)-α-pinene and turpentine could induce stronger EAG responses to males. The EAG responses of female and male adults to salicylaldehyde, (1R)-(+)-α-pinene and ocimene had no significant differences (P>0.05), but to the rest 7 volatiles had remarkable difference (P<0.01). The responses and behaviors of females to different doses of turpentine, (1R)-(+)-α-pinene, (1S)-(-)-α-pinene and salicylaldehyde, were further tested. The tested concentrations of salicylaldehyde were 0.000095, 0.00095, 0.0095, 0.095, 0.95, and 0.19 μmol/μL; the rest compounds with the same concentrations of 0.00006, 0.0006, 0.006, 0.06, 0.6, and 0.12 μmol/μL. With the concentration increased, the female EAG response to turpentine, salicylaldehyde and (1R)-(+)-α-pinene increased accordingly and kept steady when the concentration of salicylaldehyde reached 0.95 μmol/μL and those of turpentine and (1R)-(+)-α-pinene reached 0.6 μmol/μL. When the concentration were less than or equal to 0.6 μmol/μL, turpentine exhibited repellent effect to females and had the best effect at 0.6 μmol/μL (P<0.01); whereas, it showed lure effect at 0.12 μmol/μL (P>0.05). When the concentration were lower than 0.095 μmol/μL, salicylaldehyde had no obvious directional action to female (P>0.05); while higher than 0.095 μmol/μL, it exhibited repellent action (P<0.05). The females showed no distinct directional behavior to (1R)-(+)-α-pinene and (1S)-(-)-α-pinene.
