当前位置: 首页 > 文章 > 时间分辨荧光免疫层析法检测酥油中黄曲霉毒素M_1的研究 西藏农业科技 2019 (4) 37-39
Position: Home > Articles > Study on Time-Resolved Fluorescence Immunochromatography for Afaltoxin M_1 Determination in Tibetan Buttle Tibet Journal of Agricultural Sciences 2019 (4) 37-39

时间分辨荧光免疫层析法检测酥油中黄曲霉毒素M_1的研究

作  者:
魏娜;张飞龙
单  位:
西藏农牧科学院农业质量标准与检测研究所
关键词:
时间分辨荧光免疫层析法;黄曲霉毒素M_1;酥油;快速检测
摘  要:
为寻求酥油中黄曲霉毒素M_1快速准确检测方法,本研究利用时间分辨荧光免疫层析法测定酥油中黄曲霉毒素M_1含量,结果表明:该检测方法样品加标回收率为77. 6%~89. 6%,RSD <15%,与免疫亲和柱净化-HPLC法相比,相对误差<12%,该法具有简便快速、灵敏度高、重现性好等特点,可用于酥油中黄曲霉毒素M_1的快速检测。
译  名:
Study on Time-Resolved Fluorescence Immunochromatography for Afaltoxin M_1 Determination in Tibetan Buttle
作  者:
WEI Na;ZHANG Fei-long;Institutions of Agricultural Product Quality Standrad and Testing Research,Tibet Academy and Animal Husbandry Sciences;
关键词:
Time-resolved fluorescence immunochromatography;;Aflatoxin M_1;;Tibetan buttle;;Rapid assay
摘  要:
To find a rapid and accurate detection method of aflatoxin M_1 in butter,a rapid and quantitative detection method based on timeresolved fluorescence immunochromatography method was developed to analyze AFM_1 in Tibetan buttle. The results showed that the recovery rate was 77. 6 %-89. 6 %( RSD < 15 %). Compared with immuno-affinity column purification-liquid chromatography,the relative standard deviation( RSD) was less than 12 %. Time-resolved fluorescence immunochromatography method as a rapid detection of AFM_1 in Tibetan buttle had high accuracy and stability.

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