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当前位置: 首页 > 文章 > 选择不同提取剂制备斑点叉尾鮰鱼皮胶原蛋白的研究 食品科学 2008,29 (9) 321-325
Position: Home > Articles > Study on Different Extraction Methods of Collagen from Channel Catfish Skin with Different Solutions FOOD SCIENCE 2008,29 (9) 321-325

选择不同提取剂制备斑点叉尾鮰鱼皮胶原蛋白的研究

作  者：
吴缇;陈舜胜
单  位：
上海海洋大学食品学院
关键词：
斑点叉尾鮰;鱼皮;胶原蛋白;提取剂;氨基酸;黏度;变性温度
摘  要：
选择不同提取剂对斑点叉尾鮰鱼皮胶原蛋白进行制备,并对胶原蛋白进行了部分定性研究。实验结果表明,4℃条件下,鮰鱼鱼皮先用0.1mol/LNaOH浸泡6h再用2.5%NaCl浸泡6h,比单独或同时使用NaOH、NaCl能更好的去除杂蛋白,用10%的异丙醇溶液去除脂肪,用0.1mol/L柠檬酸浸提3d能较好的提取胶原蛋白,无色无味,提取率可达34.67%。获得的胶原蛋白中甘氨酸含量丰富,质量分数为21.19%,其变性温度为50.2℃。SDS-PAGE实验结果表明,提取的鮰鱼皮酸溶胶原具有较高的纯度,含有两条α链,β链含量较高。鱼皮胶原蛋白溶液具有较高的黏度,且随着蛋白浓度的增大而增大。
译  名：
Study on Different Extraction Methods of Collagen from Channel Catfish Skin with Different Solutions
作  者：
WU Ti,CHEN Shun-sheng ( College of Food Science,Shanghai Ocean University,Shanghai 200090,China)
关键词：
channel catfish; skin;collagen;extraction reagent;amino acid;viscosity; denaturation temperature
摘  要：
In this study, different reagents were chosen to extract collagen from channel catfish skin and partial characteristics of the collagen were ivestigated. The results showed that under the condition of 4 ℃, soaking the fish skins with 0.1mol/L NaOH for 6 hfollowed by with 2.5% NaCl for 6 h, can remove non-collagens better than soaking with NaOH and NaCl alone or commonly.Then the fish skins are soaked with 10% butyl alcohol to remove lipids. Finally the skins are extracted to obtain collagen with 0.1 mol/L citric acid for 3 days. The extraction rate of collagen is 34.67% and the collagen is colorless and tasteless. The collagen contains more than 21.19% glycine, and its denaturation temperature is 50.2 ℃. SDS-PAGE showed that the collagen is composed of two a chains, and the content of b chain is higher. The collagen has high viscosity, which increases as the concentration of the collagen rising .

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