作 者：
樊进茹;王世英;李佳;成志远;倪鑫;王秀伶
摘 要：
从白冠长尾雉粪样中分离出一株产氢量高的细菌菌株,命名为AUH-BG208。通过革兰氏染色和耐氧性测定,菌株AUH-BG208为革兰氏阴性兼性厌氧菌株。菌株AUH-BG208形态学及生理生化特征与克雷伯氏属一致,通过菌株16S rDNA序列分析,发现该菌株与肺炎克雷伯氏菌Klebsiella pneumoniae(AJ233420)相似性为100%。此外,对菌株AUH-BG208在不同条件下的产氢特性进行了测定。结果表明:该菌株在起始pH值为7.0,葡萄糖浓度为40 g/L,以酵母粉和硝酸钾为氮源时产氢能力最强,产氢量为1 900 mL/L。
译 名：
Isolation and identification of hydrogen-producing bacterium AUH-BG 208 and hydrogen-producing capacity under different conditions
作 者：
FAN Jin-ru,WANG Shi-ying,LI Jia,CHENG Zhi-yuan,NI Xin,WANG Xiu-ling(College of Life Sciences,Agricultural University of Hebei,Baoding 071001,China)
关键词：
hydrogen-producing bacteria;isolation;identification;hydrogen-producing property
摘 要：
One bacterium designated AUH-BG208 with higher hydrogen-producing capacity was isolated from Syrmaticus reevesii feces.Bacterial strain AUH-BG208 is a facultative gram-negative bacterium based on gram-staining and oxygen-endurance ability test.Physiological and biochemical characteristics and morphological study showed that bacterial strain AUH-BG208 had the same properties of that of genus Klebsiella.BLAST search on the GenBank revealed that the full-length of 16S rDNA gene sequence of strain AUH-BG208 has 100% similarity to that of Klebsiella pneumoniae(AJ233420).Furthermore,hydrogen-producing capacity under different conditions of bacterium AUH-BG208 was studied as well.The results showed that the optimal conditions for strain AUH-BG208 were as follows: initial pH7.0,the concentration of glucose 40 g/L,nitrogen source mixture of yeast powder and potassium nitrate.The maximum hydrogen-producing yield was 1 900 mL/L under the optimal conditions.
