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Position: Home > Articles > AFLP Diversity in the Mycorrhizal Fungi of Cymbidium Plants Acta Horticulturae Sinica 2008,35 (1) 81-86

中国兰属植物菌根真菌的AFLP多样性分析

作  者：
李潞滨;胡陶;杨凯;唐征;庄彩云;刘振静;彭镇华
单  位：
北京农学院农业应用新技术北京市重点实验室;中国林业科学研究院林业研究所国家林业局林木培育实验室
关键词：
兰属;菌根真菌;AFLP;多样性
摘  要：
对19株分离自不同地理分布和生态型的中国兰属植物菌根共生丝核菌进行了AFLP分析,12对引物组合共扩增出1869条带,其中1016条具有多态性,平均多态性比率达54.4%。AFLP分析结果采用UPGMA聚类,19株菌的相似系数在0.61～0.89之间;聚类关系显示兰花地理分布、所属种和生态类型是影响中国兰属植物菌根真菌多样性及其与兰花间专一性关系的关键性因素。地生型的春兰和云南的附生兰与其共生菌根真菌之间有着较严格的专一性关系。
译  名：
AFLP Diversity in the Mycorrhizal Fungi of Cymbidium Plants
作  者：
LI Lu-bin~1 HU Tao~1 YANG Kai~(2*) TANG Zheng~2 ZHUANG Cai-yun~1 LIU Zhen-jing~1 PENG Zhenhua~1 (1 Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China;2 Key Laboratory of Agricultural New Technology and Application in Beijing,Beijing Agricultural College,Beijing 102206,China)
关键词：
Cymbidium;;mycorrhizal fungi;;AFLP;;diversity
摘  要：
Amplified fragment length polymorphism(AFLP) was used to study the diversity of 19 repre- sentative mycorrhizal Rhizoctonia strains,which isolated from 7 species of Chinese orchids(Cymbidium) dis- tributed in different sites and belonged to different ecologic types.A high degree of diversity was found among the mycorrhizal Rhizoctonia strains.1869 bands were got by using 12 pairs of AFLP primers,and among them 1016 bands were polymorphism,the mean percentage of polymorphism was 54.4% ,which indicated that AFLP was a greatly efficient method to study the diversity of mycorrhizae of Chinese orchids (Cymbidium). The AFLP results were analyzed for similarity among strains via unweighted pair-group means cluster analysis, similarity among 19 mycorrhizal Rhizoctonia strains ranged from 0.61 to 0.89.Cluster analysis among the my- corrhizal strains showed 3 crucial factors,the distributed environments,orchids species and ecologic type of orchids,which affected the diversity of Chinese orchids(Cymbidium) symbiotic mycorrhizae and the speciality-relation between mycorrhizae and hosts.And the terrestrial orchids belong to Cymbidium goeringii and epibiosis orchids of Yunan Province showed strongly strict speciality-relation with the stains isolated from them.

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