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Position: Home > Articles > Optimization of Fermentation Conditions for Endospores Production of Antagonistic Strain Bs-0728 Journal of Henan Agricultural Sciences 2013,42 (8) 75-78

生防菌株Bs0728产芽孢条件的优化

作  者:
王佳佳;任洁;曹克强;王树桐
单  位:
河北农业大学植物保护学院
关键词:
枯草芽孢杆菌;生物防治;芽孢;发酵条件;培养基
摘  要:
前期研究证明,枯草芽孢杆菌Bs-0728对苹果再植病害具有良好的防治效果,为了提高发酵液中Bs-0728的芽孢数量,利用单因素试验和正交试验方法,通过液体摇瓶培养对Bs-0728菌株的芽孢发酵培养基和培养条件进行了筛选和优化。结果表明,Bs-0728菌株芽孢发酵的最适培养基为:玉米粉1%、花生饼粉1%、磷酸二氢钠0.4%+磷酸氢二钠0.4%;最适培养条件为:初始pH值7.5,Mn2+质量浓度200mg/L。在最优条件下发酵7d,Bs-0728菌株发酵液中的芽孢数量达到4.92×1014 cfu/mL,比初始培养条件下的芽孢数量提高了39.7倍。
译  名:
Optimization of Fermentation Conditions for Endospores Production of Antagonistic Strain Bs-0728
作  者:
WANG Jia-jia,REN Jie,CAO Ke-qiang,WANG Shu-tong*(College of Plant Protection,Agricultural University of Hebei,Baoding 071001,China)
关键词:
Bacillus subtilis;biological control;endospores;fermentation conditions;medium
摘  要:
Bacillus subtilis strain Bs-0728 has been proved to have significant control efficiency against apple replant disease previously.In order to improve the endospores yield of Bs-0728,the single factor test and orthogonal test were made to optimize the medium components and the fermentation conditions of Bs-0728.Results showed that the optimal medium composition for endospores production of Bs-0728 was 1% corn meal,1% peanut meal and 0.4% NaH2PO3+0.4% Na2HPO3,the optimal initial pH was 7.5,and the optimal concentration of Mn2+ was 200 mg/L.Under these conditions,the endospores yield of Bs-0728 achieved 4.92×1014 cfu/mL,which was 39.7 times more than that under the original fermentation condition.

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