单 位：
中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室/甘肃省动物寄生虫病重点实验室
关键词：
鸡球虫苗;LCV-2;笼养鸡;平养鸡;免疫效果
摘 要：
在试验条件下,研究了鸡球虫苗(LCV-2号)对笼养和平养鸡的免疫效果。笼养鸡免疫试验比较了笼养鸡空白对照组、攻虫对照组、免疫不攻虫组、免疫攻虫组试验期间的相对增重率、存活率、平均每克粪便卵囊(OPG)值、攻虫后第8天的肠道病变和综合抗球虫指数。结果表明,免疫不攻虫组、免疫攻虫组攻虫后没有鸡只死亡;免疫不攻虫组相对增重率最高,为97.74%,显著(P<0.05)高于免疫攻虫组和攻虫对照组(89.72%和69.85%);攻虫对照组攻虫期间平均OPG值最高,为1.26×105,而免疫攻虫组仅为5.813×104;攻虫后第8天肠道平均病变计分,空白对照组0分,攻虫对照组、免疫不攻虫组及免疫攻虫组分别为4、1、2分;4组抗球虫指数(ACI)依次为200、62.18、192.74和178.76。平养鸡免疫试验,比较了平养鸡空白对照组、不免疫不攻虫组和免疫攻虫组(2组在同1个圈)的平均OPG值、相对增重率、攻虫后第8天的肠道病变。结果表明,和免疫攻虫组鸡群一起饲养的不免疫不攻虫组鸡群增重较快,相对增重率为99.39%,而免疫攻虫组鸡群为91.67%,但差异不显著;攻虫后第8天剖检肠道平均病变计分,空白对照组0分,不免疫不攻虫组和免疫攻虫组分别为1、3分;攻虫期间免疫攻虫组鸡群平均OPG为6.025×105、不免疫不攻虫组也达3.675×105,免疫期间分别为7.5×104、3.5×104;3组ACI依次为200、193.39和175.76。从总体看,LCV-2安全性高,对笼养鸡和平养鸡都有较强的免疫效果,且对平养鸡的效果优于笼养鸡。
译 名：
Efficacy of live vaccine (LCV-2) against coccidiosis for cage-reared and floor-reared chickens
作 者：
YAN Hong-bin,TIAN Guang-fu,JIA Wan-zhong,JING Zhi-zhong,CAI Xue-peng(Key Laboratory of Veterinary Parasitology of Gansu Province,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China)
关键词：
coccidiosis;LCV-2;cages-reared chickens;floor-reared chickens;immuno-efficacy
摘 要：
Two experiments were designed to test the efficacy of LCV-2,a coccidiosis vaccine for chickens.In experiment 1,the efficacy of LCV-2 was evaluated on male broilers,Chinese Yellow strain,reared in wire cages on the basis of relative ratio of weight gain,OPG(oocysts per gram of stool),morbidity and score of intestinal tract lesions and anticoccidio index(ACI) after violent challenge.Results showed that in vaccinated challenged groups,the maximum percentage protection(survivors after challenge) against mixed species of Eimeria were 100%,and relative ratio of weight gain was observed in vaccinated non-challenged group(97.74%),which was significantly higher(P<0.05)than vaccinated challenged group(89.72%) and unvaccinated challenge group(69.85%).Furthermore,the difference between vaccinated challenged group and unvaccinated challenged group was significantly higher(P<0.05).After challenge,the average OPG of unvaccinated challenged group(1.26×105) was significantly higher(P<0.05)than vaccinated challenged groups(5.813×104),the average score of intestinal lesions were also record in unvaccined challenged groups followed by vaccined challenged groups and vaccined non-challenged group(1).The higher ACI were calculated in vaccinated non-challenged birds(192.74)and vaccinated challenged birds(178.76),which significantly higher than unvaccinated challenged birds(62.18).In experiment 2,the efficacy of LCV2 were evaluated in vaccinated challenged groups,negative control group and unvaccinated non-challenge group(which reared in one floor-pen with vaccinated challenge group together) on the basis of relative ratio of average weight gain,average OPG and intestinal lesions,too.Results showed that chickens of vaccinated non-challenged groups(with the relative ratio of weight gain 99.39%) grown faster than vaccinated challenged(with the relative ratio of weight gain 91.67%),but no significant difference was found from these two groups.After challenge,the intestinal lesions of vaccinated challenged group got worst,which score of intestinal lesions was 4,and followed by unvaccinated no-challenged group(with intestinal lesions score:1),and no intestinal lesions was observed in negative groups(with intestinal lesions score 0).Furthermore,the OPG of unvaccinated non-challenged group(3.675×105) was significantly lower(P<0.05)than vaccinated challenged group(6.025×105) after challenge,and significantly lower(P<0.01)before challenged too(7.5×104,3.5×104).The highest ACI was calculated in unvaccinated non-challenged birds(193.39),which followed by vaccinated challenged birds(175.76).From these results,it can be concluded that the vaccinated chickens,no matter of which reared in cages or in floorpens were resistant to coccidiosis with the floor-reared chickens vaccinated by LCV-2 to be better.
