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Position: Home > Articles > Use of SDS-PAGE Electrophoresis for Separation and Determination of Fibrinogen in Bovine Serum FOOD SCIENCE 2009,30 (22) 250-252

牛血清中纤维蛋白原的检测与分离

作  者:
刘爱国;吴子健;管美玲;金蓓蓓;胡静;杨桂有
单  位:
天津市食品生物技术重点实验室天津商业大学生物技术与食品科学学院
关键词:
牛血清;纤维蛋白原;检测;分离
摘  要:
通过SDS-PAGE电泳对牛血清中纤维蛋白原检测方法进行研究,确定检测参数为分离胶浓度12.5%、交联度3%、浓缩胶浓度5%。结果表明,采用4℃温度条件下、21%饱和硫酸铵溶液对牛血清中的纤维蛋白原进行分离,所得血清中纤维蛋白原的残留量仅为0.02473%。
译  名:
Use of SDS-PAGE Electrophoresis for Separation and Determination of Fibrinogen in Bovine Serum
作  者:
LIU Ai-guo,WU Zi-jian,GUAN Mei-ling,JIN Bei-bei,HU Jing,YANG Gui-you(Tianjin Key Laboratory of Food Biotechnology,College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China)
关键词:
bovine serum;fibrinogen;determination;separation
摘  要:
Fibrinogen in bovine serum was determined using tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) method,and the optimum condition were obtained as follows:separation gel concentration 12.5%,cross linking degree 3% and the concentration of spacer gel 5%.Fibrinogen was separated from bovine serum using 21% ammonium sulfate precipitation at 4 ℃,and the fibrinogen residue in bovine serum was reduced to 0.02473%.

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