当前位置: 首页 > 文章 > 以叶片为外植体建立三倍体毛白杨高效再生体系 安徽农业科学 2008,36 (32) 13979-13980+14000
Position: Home > Articles > Establishment of High Leaf-explant Regeneration System for Triploids of Chinese White Poplar Journal of Anhui Agricultural Sciences 2008,36 (32) 13979-13980+14000

以叶片为外植体建立三倍体毛白杨高效再生体系

作  者:
于桂凤;孙艳香;常世民;曹存;李晓晖
单  位:
廊坊师范学院遗传与育种研究所;廊坊市农林科学院
关键词:
三倍体毛白杨;叶片;再生体系;抗生素
摘  要:
[目的]建立三倍体毛白杨叶芽再生体系与探索抗生素对叶片再生不定芽的影响。[方法]以叶片为外植体,探索适合三倍体毛白杨遗传转化的再生条件和抗生素对叶片再生不定芽的影响。[结果]结果表明,三倍体毛白杨最适合的不定芽分化培养基配方为MS+6-BA 1.00 mg/L+NAA 0.50 mg/L;在基因工程中,40 mg/L的卡那霉素为选择性抗生素使用浓度,300 mg/L的头孢霉素为抑菌性抗生素使用浓度;再生的不定芽在MS+IBA 0.10 mg/L固体培养基中生根率可达82%。[结论]该研究为毛白杨遗传转化中适宜的培养基成分和抗生素浓度的选择提供科学依据。
译  名:
Establishment of High Leaf-explant Regeneration System for Triploids of Chinese White Poplar
作  者:
YU Gui-feng et al(Langfang Academy of Agriculture and Foresty Sciences,Langfang,Hebei 065000)
关键词:
Triploids of Chinese White Poplar;Leaf-explant;Regeneration system;Antibiotic
摘  要:
[Objective]The purpose was to establish the regeneration system and discuss the effect of antibiotics on the leaf-explant regeneration of Triploids of Chinese White Poplar.[Method]With laminae of Triploids of Chinese White Poplar as explant,the regeneration conditions that would adapt to translate of Triploids of Chinese White Poplar and the effect of antibiotics on the leaf-explant regeneration were explored.[Result]The results indicated that the optimal medium of shoot inducement for leaf-explant was MS+6-BA 1.00 mg/L+NAA 0.50 mg/L,and the optimal concentration of Kanamycin and Cefotaxime sodium in gene-engineering was 40 and 300 mg/L,respectively.The rootage rate of the reborn bud might reach 82% in MS medium with IBA 0.10 mg/L.[Conclusion]This study will provide the scientific reference for choosing the feasible medium component and concentration of antibiotics.

相似文章

计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊