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Position: Home > Articles > 黄河花园口浮游植物采集方法比较 Henan Fisheries 2021 (4) 24-28

黄河花园口浮游植物采集方法比较

作  者：
代杜娟;宗江龙;张洋;李聪;杨越;汤一帆;董静;张曼;高云霓;李学军;秦祥朝
单  位：
生态环境部黄河流域生态环境监督管理局生态环境监测与科学研究中心;河南师范大学水产学院
关键词：
黄河;泥沙;浮游植物;采样方法;
摘  要：
常规浮游植物采样方法以固定1 L水样,浓缩鉴定为主,然而这种方法较适于湖库型水体,不适于含沙量高的黄河干流浮游植物采集。为探究较适于含沙量高水体的浮游植物采集方法,作者以黄河花园口为试点,对比了不同采样方法以及不同沉降时间、不同采集网孔径对黄河花园口浮游植物采集的影响。研究结果表明:（1）常规取水样浓缩与35μm滤网过滤浓缩相比,其采集到的浮游藻类种类数无显著差异,然而采集到的种类有不重叠部分,也就是说二者可互补提高采集浮游藻类的种类数;（2）相较于10μm滤网,35μm滤网过滤能鉴定到更多的浮游藻类,且无论是35μm还是10μm滤网过滤前,对含沙量高的水样进行短时间沉淀,有助于提高鉴定浮游藻类种类数,以沉降1 min内为宜;（3）虽然滤网过滤可提高采集鉴定浮游植物的种类数,但是若需要浮游植物定量数据,常规浓缩鉴定更加适合。据此,通过该试点调查分析,作者认为对于含沙量高的水体可借助常规采样和35μm滤网共同取样提高浮游植物种类数,然而浮游植物的定量数据仍需以常规浓缩鉴定方法为主,若实际水体浮游藻类生物量偏低,可适量提高常规采样体积。
作  者：
Dai Dujuan;Zong Jianglong;Zhang Yang;Li Cong;Yang Yue;Tang Yifan;Dong Jing;Zhang Man;Gao Yunni;Li Xuejun;Qin Xiangchao;College of Fisheries, Henan Normal University;Research Center for Ecological Environment Monitoring and Scientific, Yellow River Basin Ecological Environment Administration, Ministry of Ecological Environment;
关键词：
Yellow River;;sediment;;phytoplankton;;sampling methods
摘  要：
The conventional phytoplankton sampling method is based on a fixed 1 L water sample and following concentration and identification. However, this method is more suitable for phytoplankton survey in lakes or reservoirs, and is not suitable for the collection of phytoplankton in the mainstream of the Yellow River with high amount of sands. In order to explore the phytoplankton collection methods that are more suitable for water bodies with high amount of sands, the authors took the Huayuankou sites of Yellow River as a pilot and compared the phytoplankton survey results by different sampling methods, different settlement time and different-size collection net. The results are as follows:(1) Compared with the concentration by 35 μm filter, there was no significant difference in the species number of phytoplankton collected by conventional methods. However, the collected and identified species had non-overlapping parts, which meant that the two methods could complement each other, thus increasing the total species number of the survey phytoplankton;(2) Compared with the 10 μm filter, more species were identified under 35 μm filter, and short period(within one minute) of samples precipitation was helpful prior to the 35 μm and 10 μm filtering for the water samples with high amount of sands, which could largely increase the species number the surveyed phytoplankton;(3) Although filter filtering can increase species number for phytoplankton identification, if quantitative data of phytoplankton is required, conventional concentration identification is more suitable and essentially required for the phytoplanton quantitative analysis(phytoplankton biomass and abundance). Based on these results, the authors suggested that for the phytoplankton survey of the water body with high amount of sands, conventional sampling and 35 μm filter sampling can be applied simultaneously to increase the surveyed species number of phytoplankton. However, the quantitative data of phytoplankton still needs to be based on conventional enrichment identification methods. If the phytoplankton biomass in the water body is quite low, the conventional sampling volume can be increased appropriately.

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