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当前位置: 首页 > 文章 > 东方欧鳊腐皮病病原菌的分离鉴定及药敏试验的研究 水产养殖 2013,34 (10) 1-4
Position: Home > Articles > Isolation,identification and drug sensitive test on pathogens of skin ulcer from European bream Journal of Aquaculture 2013,34 (10) 1-4

东方欧鳊腐皮病病原菌的分离鉴定及药敏试验的研究

作  者：
成沛;张凤萍;李胜忠;关秀芝;阳忠财
单  位：
新疆农业大学动物科学学院;安宁市水产局
关键词：
东方欧鳊;腐皮病;病原菌;药敏试验
摘  要：
为鉴定东方欧鳊腐皮病的病原菌,采用API 32E细菌自动鉴定系统,对东方欧鳊体表病灶部位进行分离、纯化和鉴定,并进行人工感染试验和药敏试验。结果表明:菌株革兰氏阴性,杆状,菌体大小(0.8~1.8)×(0.5~0.7)μm。βN乙酰葡萄糖胺、α-麦芽糖和L天冬氨酸芳胺酶等反应呈阳性。肌醇、L-阿拉伯糖和尿素酶等呈阴性。注射感染浓度1.5×108和1.5×105cfu/mL实验组,48 h分别死亡7尾和2尾。1.5×104cfu/mL实验组和对照组没有死亡发生。半致死浓度LD50为7.1×106cfu/mL。分离菌株CP01为腐败希瓦氏菌(Shewanella putrefaciens)。菌株CP01对氟哌酸、恩诺沙星和强力霉素敏感,对青霉素和先锋必素不敏感。
译  名：
Isolation,identification and drug sensitive test on pathogens of skin ulcer from European bream
作  者：
Cheng Pei;Zhang Fengping;Li Shengzhong;Guan Xiuzhi;Yang zhongcai;College of Animal Science,Xinjiang Agriculture University;Fishery Bureau of Anning City;
关键词：
European bream;;pathogen;;skin ulcer;;drug sensitive test
摘  要：
A bacterium(CP01) was isolated from the lesion surface,with the methods of bacterial conventional separation,in order to make sure the skin ulcer pathogen of the European bream(Abramis brama orientalis),providing a scientific proof for its diagnostic,prevention and treatment.The artificial infection experiment,physiological and biochemical characteristics and drug sensitive test were studied.The results showed that:the strain CP01 was in the shape of rod,about 0.8~1.8 μm long,and of Gram-negative.βN acetyl glucosamine,alpha-maltose,and L-aspartic acid aryl amine enzyme reaction were positive.Inositol,L-arabinose and urea enzymes were negative.The died number of 1.5×108cfu/mL concentration group of Injection infection was seven and the 1.5×104cfu/mL group is two.No death occured in the control group.The LD50of the strain CP01 to the adults of European bream was 7.1×106cfu/mL.The strain CP01 was Shewanella putrefaciens.The antibiotic sensitivity test showed that the strain CP01 was sensitive to 3 drugs including norfloxacin,enrofloxacin and doxycycline,insensitive to penicillin and cefoperazonum.

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