当前位置: 首页 > 文章 > OsBTF3转基因水稻对病原茵侵染的反应和防卫基因的表达分析 植物病理学报 2012,42 (2) 169-175
Position: Home > Articles > Responses and expression of defense genes of transgenic rice with over-expressed or RNAi-silenced OsBTF3 gene upon bacterial infection Acta Phytopathologica Sinica 2012,42 (2) 169-175

OsBTF3转基因水稻对病原茵侵染的反应和防卫基因的表达分析

作  者:
李广旭;陈华民;吴茂森;何晨阳
单  位:
植物病虫害生物学国家重点实验室
关键词:
水稻;OsBTF3;转基因;感病性;防卫基因表达;rice; OsBTF3 ; transgenic; disease susceptibility; defense gene expression
摘  要:
为了阐明OsBTF3基因在水稻对白叶枯病菌(Xanthomonas oryzae pvoryzae,Xoo)和细菌性条斑病菌(Xoryzae pvoryzicola,Xoc)侵染抗/感反应中的功能,本研究以OsBTF3过量表达和RNAi转基因株系为材料,比较分析了T.代株系对病菌侵染的抗感病反应,定量测定了防卫基因的表达动态.结果表明,与野生型对照株相比,无论过量表达株系,还是RNAi株系对Xoo和Xoc侵染均表现为更加感病的反应;经Xoo接种后,过量表达和RNAi株系OE37和RNAi株系R130防卫基因的表达发生了不同程度的变化.因此,OsBTF3增量/减量表达对水稻抗/感病性具有重要的调控作用,这种作用可能并不依赖于水杨酸(SA)和茉莉酸(JA)介导的信号途径.
译  名:
Responses and expression of defense genes of transgenic rice with over-expressed or RNAi-silenced OsBTF3 gene upon bacterial infection
摘  要:
To reveal the functions of OsBTF3 gene in rice in response to infection by Xanthomonas oryzae pvs. oryzae and oryzicola, the responses and expression of defense genes of transgenic rice with over-expressed or RNAi- silenced OsBTF3 were comparably analyzed. The results showed that more susceptible phenotypes were found either in transgenic rice with over-expressed or RNAi-silenced OsBTF3 than the control of wildtype plants. In addition, the changes in expression of selected defense genes induced by Xoo were observed both in the transgenic rice lines and the control plants. Therefore, over- or down-expression of OsBTF3 regulates the disease resistance/susceptibili- ty in rice, which is independent on SA- or JA-mediated signaling pathways.

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