当前位置: 首页 > 文章 > 农杆菌介导的花生Δ~(12)脂肪酸脱氢酶基因AhFAD2 RNAi抑制表达遗传转化研究 中国油料作物学报 2007,29 (4) 409-415+419
Position: Home > Articles > Transformation of RNAi suppressed expression vector containing of Δ~(12) fatty acid desaturase gene via Agrobacterium infection in Arachis hypogaea L. Chinese Journal of Oil Crop Sciences 2007,29 (4) 409-415+419

农杆菌介导的花生Δ~(12)脂肪酸脱氢酶基因AhFAD2 RNAi抑制表达遗传转化研究

作  者:
张小茜;单雷;唐桂英;滕娜;毕玉平
单  位:
山东省农业科学院高新技术研究中心山东省作物与畜禽品种改良生物技术重点实验室
关键词:
花生;农杆菌介导法;Δ12脂肪酸脱氢酶基因;RNA干扰
摘  要:
以成熟花生种子萌动4d的上胚轴为受体材料,采用农杆菌介导的转化方法,将倒位重复Δ12脂肪酸脱氢酶基因AhFAD2片段分别与CaMV35S启动子和种子特异性表达的大豆凝集素启动子相连导入花生。经PCR检测证实含有AhFAD2倒位重复基因片段的RNAi抑制表达框架已成功转入花生。
译  名:
Transformation of RNAi suppressed expression vector containing of Δ~(12) fatty acid desaturase gene via Agrobacterium infection in Arachis hypogaea L.
作  者:
ZHANG Xiao-qian1,2,SHAN Lei1*,TANG Gui-ying1,TENG Na1,BI Yu-ping1,2* (1.High-Tech Research Center,Shangdong Academy of Agricultural Science;Key Laboratory for Genetic Improvement of Crop,Animal and Poultry of Shandong Province,Jinan 250100,China;2.Life Science College of Shandong Normal's University,Jinan 250014,China)
关键词:
Arachis hypogaea L.,Agrobecterium infection,Δ12fatty acid desaturase gene,RNA interfere
摘  要:
Using hypocotyl as recipients,the inverted repeat sequence of partial Δ12fatty acid desaturase gene AhFAD2 with strong seed specific soybean lectin promoter or CaMV35S promoter,was introduced into Arachis hypogaea L.via Agrobecterium infection.Transgenic peanuts containing the inverted repeat structure were confirmed by PCR.

相似文章

计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊